人甲型流感病毒A/WSN/33聚合酶3基因的完整核苷酸序列
Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33.
作者信息
Kaptein J S, Nayak D P
出版信息
J Virol. 1982 Apr;42(1):55-63. doi: 10.1128/JVI.42.1.55-63.1982.
Abstract
The complete nucleotide sequence of polymerase 3 (P3) gene of a human influenza virus (A/WSN/33) has been determined using cDNA clones except for the last 11 nucleotides which were obtained by direct RNA sequencing. The WSN P3 gene contains 2,341 nucleotides and codes for a protein of 759 amino acids (molecular weight 85,800). The WSN P3 protein, as deduced from the plus-strand DNA sequence, is basic and enriched in positively charged amino acids. In addition, it contains clusters of basic amino acids which may provide sites for the interaction of P3 protein with the capped primer, template, and/or other polymerase proteins during the transcriptive and replicative processes of influenza viral RNA.
摘要
利用cDNA克隆已确定了一株人流感病毒（A/WSN/33）聚合酶3（P3）基因的完整核苷酸序列，但最后11个核苷酸是通过直接RNA测序获得的。WSN P3基因包含2341个核苷酸，编码一个由759个氨基酸组成的蛋白质（分子量85800）。从正链DNA序列推导得出的WSN P3蛋白呈碱性，富含带正电荷的氨基酸。此外，它还含有碱性氨基酸簇，这些碱性氨基酸簇可能在流感病毒RNA的转录和复制过程中为P3蛋白与带帽引物、模板和/或其他聚合酶蛋白的相互作用提供位点。
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