Baez M, Taussig R, Zazra J J, Young J F, Palese P, Reisfeld A, Skalka A M
Nucleic Acids Res. 1980 Dec 11;8(23):5845-58. doi: 10.1093/nar/8.23.5845.
The nucleotide sequence of the NS gene of the human influenza virus A/PR/8/34 was determined and found to be the same length (890 nucleotides) as the NS gene of another human influenza virus A/Udorn/72 and of the avian isolate A/FPV/Rostock/34. Comparison of the sequences of the NS genes of the two human influenza viruses shows an 8.9% difference whereas the NS gene of the avian isolate differs by only 8% from that of the human strain A/PR/8/34. The extensive sequence similarity among these three genes does not support the notion of species specific homology groups among NS genes of avian and human influenza virus strains. The primary sequence of the A/PR/8/34 NS gene is consistent with the findings that the influenza virus NS gene may code for two overlapping polypeptides. In addition, an open reading frame potentially coding for a polypeptide 167 amino acids in length was found in the negative strand RNA of the A/PR/8/34 virus NS gene.
测定了人流感病毒A/PR/8/34的NS基因的核苷酸序列，发现其长度（890个核苷酸）与另一种人流感病毒A/Udorn/72以及禽源分离株A/FPV/Rostock/34的NS基因相同。两种人流感病毒NS基因序列的比较显示存在8.9%的差异，而禽源分离株的NS基因与人类毒株A/PR/8/34的NS基因仅相差8%。这三个基因之间广泛的序列相似性并不支持禽流感病毒株和人流感病毒株的NS基因存在物种特异性同源组的观点。A/PR/8/34 NS基因的一级序列与流感病毒NS基因可能编码两种重叠多肽的发现一致。此外，在A/PR/8/34病毒NS基因的负链RNA中发现了一个潜在编码长度为167个氨基酸的多肽的开放阅读框。
