Sahib Hayder B, Kathum Omer Abid, Alanee Rafal Shakeeb, Jawad Rehab A M, Al-Shammari Ahmed Majeed
Al-Nahrain University, College of Pharmacy, Pharmacology Department, Baghdad, Iraq.
Al-Nahrain University, Biotechnology Research Center, Baghdad, Iraq.
Adv Virol. 2022 Jun 8;2022:1575605. doi: 10.1155/2022/1575605. eCollection 2022.
Cytokine storm is one of the causative deaths in a patient with severe acute respiratory syndrome. This study aimed at evaluating the prophylaxis effect of quercetin complexes with zinc and buffered ascorbic acid upon cytokine storm induction in mice and identifying the complex's acute toxicity. Mice were randomly divided into three groups: group A, control group, received 0.9% normal saline; group B received 100 mg/kg of the complex one hour before lipopolysaccharide (LPS) administration; and group C received the LPS IP 5 mg/kg. Then, levels of interleukin 1 and interleukin 6 were measured in the serum, and lung and kidney tissues were investigated for any changes that may have happened. Thirty mice were used to investigate the acute toxicity; mice were distributed into six groups: one control group and five treated groups; then several serial dilutions from the complex have been prepared for different concentrations from 5 g/kg to 0.312 g/kg. The animals were observed for 14 days. The LD was deduced by the straight-line equation calculated from the dose-response curve. The results in this study showed that group A had no significant tissue change. LPS group C showed tissue damage in the lung and kidney, which significantly prevented by the pretreated complex in group B. Moreover, the complex's acute toxicity value (LD) was 655 mg/kg. In conclusion, the complex has significantly ameliorated LPS-induced acute lung and kidney injury, largely through suppression of inflammation; the large lethal dose value may make the complex have a promising therapeutic effect in the prevention of cytokine storm.
细胞因子风暴是重症急性呼吸综合征患者致死原因之一。本研究旨在评估槲皮素与锌及缓冲抗坏血酸的复合物对小鼠细胞因子风暴诱导的预防作用，并确定该复合物的急性毒性。将小鼠随机分为三组：A组为对照组，给予0.9%生理盐水；B组在给予脂多糖（LPS）前1小时给予100mg/kg该复合物；C组腹腔注射5mg/kg LPS。然后，检测血清中白细胞介素1和白细胞介素6的水平，并研究肺和肾组织是否发生任何变化。使用30只小鼠研究急性毒性；将小鼠分为六组：一组对照组和五组处理组；然后针对从5g/kg到0.312g/kg的不同浓度制备该复合物的几种系列稀释液。观察动物14天。通过从剂量-反应曲线计算的直线方程推导出半数致死量（LD）。本研究结果表明，A组无明显组织变化。LPS处理的C组在肺和肾中出现组织损伤，而B组预先处理的复合物可显著预防这种损伤。此外，该复合物的急性毒性值（LD）为655mg/kg。总之，该复合物通过抑制炎症反应，显著改善了LPS诱导的急性肺和肾损伤；较大的致死剂量值可能使该复合物在预防细胞因子风暴方面具有有前景的治疗效果。
