Diabetes Foundation (India), Safdarjung Development Area, New Delhi, 110016, India; National Diabetes Obesity and Cholesterol Foundation (N-DOC), Safdarjung Development Area, New Delhi, 110016, India; Department of Pulmonary, Critical Care and Sleep Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India; Fortis C-DOC Center of Excellence for Diabetes, Metabolic Diseases, and Endocrinology, B-16, Chirag Enclave, New Delhi, India.
Diabetes Foundation (India), Safdarjung Development Area, New Delhi, 110016, India; National Diabetes Obesity and Cholesterol Foundation (N-DOC), Safdarjung Development Area, New Delhi, 110016, India; Fortis C-DOC Center of Excellence for Diabetes, Metabolic Diseases, and Endocrinology, B-16, Chirag Enclave, New Delhi, India.
Diabetes Metab Syndr. 2020 May-Jun;14(3):175-180. doi: 10.1016/j.dsx.2020.02.011. Epub 2020 Feb 21.
Transcription factor 7 like 2 (TCF7L-2) polymorphism has been associated with adipocyte metabolism and insulin resistance. Genetic investigations of non-alcoholic fatty liver disease (NAFLD) with obesity, type 2 diabetes mellitus (T2DM) and atherosclerosis are unknown. This study was designed to investigate the association of rs7903146 (C/T) polymorphism of TCF7L-2 gene with non-alcoholic fatty liver disease (NAFLD) in Asian Indians.
In this case-control study 162 non-diabetic subjects with NAFLD and 173 body mass index (BMI)-matched controls without NAFLD were recruited. Abdominal ultrasound, clinical and biochemical investigations, fasting insulin levels and value of homeostasis model assessment of insulin resistance (HOMA-IR) was measured. Single nucleotide polymorphism rs7903146 (C/T) was genotyped by polymerase chain reaction-restriction fragment length polymorphisms.
The distribution of rs7903146 (C/T) alleles, the dominant model (CT + TT) and higher frequency (31%) of C/T genotype were significantly associated with NAFLD. C/T genotype of TCF7L2 gene was associated with significantly higher levels of BMI (p = 0.02), abdominal obesity (p < 0.05), fasting blood glucose (p = 0.05), hepatic transaminases (p < 0.05) and markers of insulin resistance (p < 0.05) in subjects with NAFLD. Using a multivariate analysis after adjusting for age and sex, TCF7L2 polymorphism was independently associated with presence of NAFLD [(OR: 3.234 (95% CI: 1.219-4.160, p = 0.002)].
TCF7L2 (C/T) gene was Independently associated with NAFLD in Asian Indians.
转录因子 7 样 2（TCF7L-2）多态性与脂肪细胞代谢和胰岛素抵抗有关。关于非酒精性脂肪性肝病（NAFLD）与肥胖、2 型糖尿病（T2DM）和动脉粥样硬化的遗传研究尚不清楚。本研究旨在探讨 TCF7L-2 基因 rs7903146（C/T）多态性与亚洲印度人非酒精性脂肪性肝病（NAFLD）的相关性。
在这项病例对照研究中，共招募了 162 名非糖尿病合并 NAFLD 的患者和 173 名 BMI 匹配的无 NAFLD 的对照组。进行了腹部超声、临床和生化检查、空腹胰岛素水平以及稳态模型评估的胰岛素抵抗（HOMA-IR）值的测量。采用聚合酶链反应-限制性片段长度多态性方法检测单核苷酸多态性 rs7903146（C/T）。
rs7903146（C/T）等位基因、显性模型（CT+TT）和更高的 C/T 基因型频率（31%）与 NAFLD 显著相关。TCF7L2 基因的 C/T 基因型与 NAFLD 患者的 BMI（p=0.02）、腹部肥胖（p<0.05）、空腹血糖（p=0.05）、肝转氨酶（p<0.05）和胰岛素抵抗标志物（p<0.05）显著升高相关。在调整年龄和性别后进行多变量分析，TCF7L2 多态性与 NAFLD 的存在独立相关[比值比（OR）：3.234（95%置信区间：1.219-4.160，p=0.002）]。
TCF7L2（C/T）基因与亚洲印度人的 NAFLD 独立相关。
