大鼠和小鼠精子中支链氨基酸转氨酶及乳酸脱氢酶C4的亚细胞定位
Subcellular localization of branched-chain amino acid aminotransferase and lactate dehydrogenase C4 in rat and mouse spermatozoa.
作者信息
Montamat E E, Vermouth N T, Blanco A
机构信息
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
出版信息
Biochem J. 1988 Nov 1;255(3):1053-6. doi: 10.1042/bj2551053.
Abstract
Spermatozoa isolated from rat and mouse epididymes show a relatively high branched-chain amino acid aminotransferase (leucine aminotransferase, EC 2.6.1.6) activity. There is a significant reduction of leucine aminotransferase and of the isoenzyme C4 of lactate dehydrogenase (EC 1.1.1.27) in the gametes during their epididymal transit. Studies of patterns of liberation of the leucine aminotransferase and of the lactate dehydrogenase C4 from intact spermatozoa, treated with increasing concentrations of digitonin, indicate that both enzymes have the same dual subcellular location, i.e. in the cytosol and in the mitochondria.
摘要
从大鼠和小鼠附睾中分离出的精子显示出相对较高的支链氨基酸转氨酶（亮氨酸转氨酶，EC 2.6.1.6）活性。在配子附睾转运过程中，亮氨酸转氨酶和乳酸脱氢酶（EC 1.1.1.27）同工酶C4显著减少。用浓度递增的洋地黄皂苷处理完整精子，对亮氨酸转氨酶和乳酸脱氢酶C4释放模式的研究表明，这两种酶具有相同的双重亚细胞定位，即在细胞质和线粒体中。
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