大鼠肝脏丝氨酸 - 丙酮酸氨基转移酶的亚细胞分布
The subcellular distribution of rat liver serine-pyruvate aminotransferase.
作者信息
Rowsell K V, Al-Naama L M, Benett P
出版信息
Biochem J. 1982 Feb 15;202(2):483-90. doi: 10.1042/bj2020483.
Abstract
- The subcellular distribution of L-serine-pyruvate aminotransferase activity in rat liver was investigated. About 80% was recovered from cell-free homogenates in a 'total-particles' fraction and the remainder in the cytosol. 2. Subfractionation of the particles by differential sedimentation and on sucrose density gradients showed a distribution for serine-pyruvate aminotransferase activity closely matching that observed for mitochondrial marker enzymes. 3. A study of the solubilization of enzymes from combined subcellular particles by digitonin at various concentrations also indicated a common subcellular location for serine-pyruvate aminotransferase and established mitochondrial enzymes. 4. The increase in liver serine-pyruvate amino-transferase activity induced by glucagon injection was accounted for as an increased mitochondrial activity.
摘要
- 对大鼠肝脏中L-丝氨酸-丙酮酸氨基转移酶活性的亚细胞分布进行了研究。约80%从无细胞匀浆中回收于“总颗粒”部分，其余存在于胞质溶胶中。2. 通过差速沉降和蔗糖密度梯度对颗粒进行亚分级分离，结果显示丝氨酸-丙酮酸氨基转移酶活性的分布与线粒体标记酶的分布密切匹配。3. 对不同浓度洋地黄皂苷从合并的亚细胞颗粒中溶解酶的研究也表明，丝氨酸-丙酮酸氨基转移酶和已确定的线粒体酶具有共同的亚细胞定位。4. 胰高血糖素注射诱导的肝脏丝氨酸-丙酮酸氨基转移酶活性增加被认为是线粒体活性增加所致。
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