一种用于大鼠肝细胞膜氨基酸转运系统功能重建的快速方法。A系统的部分纯化。
A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.
作者信息
Quesada A R, McGivan J D
机构信息
Department of Biochemistry, University of Bristol, U.K.
出版信息
Biochem J. 1988 Nov 1;255(3):963-9. doi: 10.1042/bj2550963.
Abstract
A rapid method for the functional reconstruction of amino acid transport from liver plasma-membrane vesicles using the neutral detergent decanoyl-N-glucamide ('MEGA-10') is described. The method is a modification of that previously employed in this laboratory for reconstitution of amino acid transport systems from kidney brush-border membranes [Lynch & McGivan (1987) Biochem. J. 244, 503-508]. The transport activities termed 'System A', 'System N', and 'System L' are all reconstituted. The reconstitution procedure is rapid and efficient and is suitable as an assay for transport activity in studies involving membrane fractionation. By using this reconstitution procedure, System A transport activity was partially purified by lectin-affinity chromatography.
摘要
本文描述了一种使用中性去污剂癸酰-N-葡萄糖酰胺（“MEGA-10”）从肝血浆膜囊泡中快速进行氨基酸转运功能重建的方法。该方法是对本实验室先前用于从肾刷状缘膜重建氨基酸转运系统的方法的改进[Lynch & McGivan（1987年）《生物化学杂志》244卷，503 - 508页]。被称为“A系统”、“N系统”和“L系统”的转运活性均得以重建。重建过程快速且高效，适用于涉及膜分级分离研究中的转运活性测定。通过使用这种重建方法，A系统转运活性通过凝集素亲和色谱法进行了部分纯化。
相似文献
1
A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.一种用于大鼠肝细胞膜氨基酸转运系统功能重建的快速方法。A系统的部分纯化。
Biochem J. 1988 Nov 1;255(3):963-9. doi: 10.1042/bj2550963.
2
A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes.一种用于重构牛肾刷状缘膜中钠依赖性中性氨基酸转运的快速方法。
Biochem J. 1987 Jun 15;244(3):503-8. doi: 10.1042/bj2440503.
3
Substrate-specificity of glutamine transporters in membrane vesicles from rat liver and skeletal muscle investigated using amino acid analogues.使用氨基酸类似物研究大鼠肝脏和骨骼肌膜囊泡中谷氨酰胺转运体的底物特异性。
Biochem J. 1991 Aug 15;278 ( Pt 1)(Pt 1):105-11. doi: 10.1042/bj2780105.
4
Sensitivity of system A and ASC transport activities to thiol-group-modifying reagents in rat liver plasma-membrane vesicles. Evidence for a direct binding of N-ethylmaleimide and iodoacetamide on A and ASC carriers.大鼠肝血浆膜囊泡中系统A和ASC转运活性对巯基修饰试剂的敏感性。N-乙基马来酰亚胺和碘乙酰胺与A和ASC载体直接结合的证据。
Biochem J. 1990 Oct 15;271(2):297-303. doi: 10.1042/bj2710297.
5
Reconstitution into liposomes of the B degrees -like glutamine-neutral amino acid transporter from renal cell plasma membrane.将来自肾细胞质膜的B°样谷氨酰胺中性氨基酸转运体重组到脂质体中。
Biochim Biophys Acta. 2008 Oct;1778(10):2258-65. doi: 10.1016/j.bbamem.2008.05.011. Epub 2008 Jun 3.
6
Reconstitution of neutral amino acid transport from partially purified membrane components from Ehrlich ascites tumor cells.从艾氏腹水瘤细胞的部分纯化膜成分中重建中性氨基酸转运。
J Supramol Struct. 1977;7(3-4):481-7. doi: 10.1002/jss.400070317.
7
Reconstitution into liposomes of the glutamine/amino acid transporter from renal cell plasma membrane: functional characterization, kinetics and activation by nucleotides.肾细胞质膜谷氨酰胺/氨基酸转运体在脂质体中的重组：功能特性、动力学及核苷酸激活作用
Biochim Biophys Acta. 2004 Dec 15;1667(2):122-31. doi: 10.1016/j.bbamem.2004.09.007.
8
Comparison of the effects of certain thiol reagents on alanine transport in plasma membrane vesicles from rat liver and their use in identifying the alanine carrier.某些硫醇试剂对大鼠肝脏质膜囊泡中丙氨酸转运的影响比较及其在鉴定丙氨酸载体中的应用
Biochem J. 1983 Aug 15;214(2):489-95. doi: 10.1042/bj2140489.
9
Sulfhydryl group involvement in the modulation of neutral amino acid transport in thymocyte membrane vesicles.巯基参与胸腺细胞膜囊泡中中性氨基酸转运的调节。
J Cell Physiol. 1981 Feb;106(2):279-82. doi: 10.1002/jcp.1041060214.
10
Substrate-dependent adaptive regulation and trans-inhibition of System A-mediated amino acid transport. Studies using rat hepatoma plasma membrane vesicles.系统A介导的氨基酸转运的底物依赖性适应性调节和反式抑制。使用大鼠肝癌质膜囊泡的研究。
Biochim Biophys Acta. 1990 Mar;1022(3):325-32. doi: 10.1016/0005-2736(90)90281-r.
引用本文的文献
1
Amino acid transport system A resembles system N in sequence but differs in mechanism.氨基酸转运系统A在序列上与系统N相似，但机制不同。
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7715-20. doi: 10.1073/pnas.140152797.
2
Reconstitution studies of amino acid transport system L in rat erythrocytes.大鼠红细胞中氨基酸转运系统L的重组研究。
Biochem J. 1993 Jun 15;292 ( Pt 3)(Pt 3):655-60. doi: 10.1042/bj2920655.
3
Regulatory and molecular aspects of mammalian amino acid transport.哺乳动物氨基酸转运的调节及分子层面
Biochem J. 1994 Apr 15;299 ( Pt 2)(Pt 2):321-34. doi: 10.1042/bj2990321.
4
Evidence for a regulatory protein involved in the increased activity of system A for neutral amino acid transport in osmotically stressed mammalian cells.有证据表明，在渗透应激的哺乳动物细胞中，存在一种调节蛋白参与了系统A对中性氨基酸转运活性的增强。
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9569-73. doi: 10.1073/pnas.91.20.9569.
5
Expression of rat liver Na+/L-alanine co-transport in Xenopus laevis oocytes. Effect of glucagon in vivo.大鼠肝脏Na⁺/L-丙氨酸共转运体在非洲爪蟾卵母细胞中的表达。体内胰高血糖素的作用。
Biochem J. 1990 Aug 15;270(1):189-95. doi: 10.1042/bj2700189.
6
Sensitivity of system A and ASC transport activities to thiol-group-modifying reagents in rat liver plasma-membrane vesicles. Evidence for a direct binding of N-ethylmaleimide and iodoacetamide on A and ASC carriers.大鼠肝血浆膜囊泡中系统A和ASC转运活性对巯基修饰试剂的敏感性。N-乙基马来酰亚胺和碘乙酰胺与A和ASC载体直接结合的证据。
Biochem J. 1990 Oct 15;271(2):297-303. doi: 10.1042/bj2710297.
7
Functional reconstitution of the hepatic system N amino acid transport activity.肝脏系统N氨基酸转运活性的功能重建。
Biochem J. 1991 Feb 15;274 ( Pt 1)(Pt 1):97-101. doi: 10.1042/bj2740097.
8
L-glutamine transport in native vesicles isolated from Ehrlich ascites tumor cell membranes.从艾氏腹水肿瘤细胞膜分离出的天然囊泡中的L-谷氨酰胺转运
J Bioenerg Biomembr. 1991 Aug;23(4):689-97. doi: 10.1007/BF00785818.
9
Sodium-dependent L-serine transport in plasma membrane vesicles isolated from Ehrlich cells by two-phase compartmentation.通过两相分离从艾氏腹水癌细胞中分离出的质膜囊泡中的钠依赖性L-丝氨酸转运。
J Membr Biol. 1991 Sep;123(3):247-54. doi: 10.1007/BF01870407.
10
Reconstitution and identification of the major Na(+)-dependent neutral amino acid-transport protein from bovine renal brush-border membrane vesicles.牛肾刷状缘膜囊泡中主要的钠离子依赖性中性氨基酸转运蛋白的重组与鉴定。
Biochem J. 1992 Jan 1;281 ( Pt 1)(Pt 1):95-102. doi: 10.1042/bj2810095.
本文引用的文献
1
ON THE MECHANISM OF ACTION OF PHOSPHOLIPASE A.关于磷脂酶A的作用机制
Biochem J. 1963 Sep;88(3):414-23. doi: 10.1042/bj0880414.
2
Amino acid transport in plasma-membrane vesicles from rat liver. Characterization of L-alanine transport.大鼠肝脏质膜囊泡中的氨基酸转运。L-丙氨酸转运的特性
Eur J Biochem. 1980 Apr;105(2):217-24. doi: 10.1111/j.1432-1033.1980.tb04492.x.
3
4
Amino acid-dependent sodium transport in plasma membrane vesicles from rat liver.大鼠肝脏质膜囊泡中氨基酸依赖的钠转运
J Membr Biol. 1981;62(3):231-7. doi: 10.1007/BF01998168.
5
N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry.N-D-葡萄糖-N-甲基链烷酰胺化合物，一类用于膜生物化学的新型非离子洗涤剂。
Biochem J. 1982 Nov 1;207(2):363-6. doi: 10.1042/bj2070363.
6
Amino acid transport in isolated rat hepatocytes.分离的大鼠肝细胞中的氨基酸转运
J Membr Biol. 1982;69(1):1-12. doi: 10.1007/BF01871236.
7
A simple and efficient method for reconstitution of amino acid and glucose transport systems from Ehrlich ascites cells.一种从艾氏腹水癌细胞重建氨基酸和葡萄糖转运系统的简单有效方法。
Arch Biochem Biophys. 1984 Jun;231(2):355-65. doi: 10.1016/0003-9861(84)90398-9.
8
9
Comparison of the effects of certain thiol reagents on alanine transport in plasma membrane vesicles from rat liver and their use in identifying the alanine carrier.某些硫醇试剂对大鼠肝脏质膜囊泡中丙氨酸转运的影响比较及其在鉴定丙氨酸载体中的应用
Biochem J. 1983 Aug 15;214(2):489-95. doi: 10.1042/bj2140489.
10
Amino acid transport in plasma membrane vesicles from isolated rat liver parenchymal cells.来自分离的大鼠肝脏实质细胞的质膜囊泡中的氨基酸转运
Biochim Biophys Acta. 1980 Aug 4;600(2):577-80. doi: 10.1016/0005-2736(80)90458-7.
Zotero 插件