一种用于重构牛肾刷状缘膜中钠依赖性中性氨基酸转运的快速方法。
A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes.
作者信息
Lynch A M, McGivan J D
机构信息
Department of Biochemistry, Medical School, Bristol, U.K.
出版信息
Biochem J. 1987 Jun 15;244(3):503-8. doi: 10.1042/bj2440503.
Abstract
- A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide ('MEGA-10') was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins.
摘要
- 本文描述了一种简单快速的方法，用于重构牛肾刷状缘膜中依赖钠离子的中性氨基酸转运活性。2. 使用中性去污剂癸酰-N-甲基葡糖酰胺（“MEGA-10”）溶解膜蛋白。这避免了长时间透析步骤的必要性。3. 这些囊泡中氨基酸转运的特性与在天然膜中观察到的相似。4. 这在最终鉴定和分离氨基酸转运蛋白方面应该是一个有用的程序。
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