Mazoyer E, Ebbe S, Dalal K, Leven R, Mazoyer B, Carpenter D, Yee T, Brennan K
Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Atherosclerosis. 1988 Nov;74(1-2):23-32. doi: 10.1016/0021-9150(88)90187-6.
Hypercholesterolemia (HC = hypercholesterolemia or hypercholesterolemic) was produced in rabbits by feeding them diets supplemented with cholesterol and peanut oil. Platelet counts and volumes, white cell counts, reticulocyte counts, and hematocrits were determined at intervals for 8-12 weeks in blood from HC animals and controls on a normal rabbit diet. Microthrombocytosis was a consistent occurrence in the presence of HC, developing as early as 2 weeks into the diet. Microthrombocytosis was generally associated with normal platelet counts, but mild thrombocytosis occurred late in the diet at the time of the highest levels of serum cholesterol (greater than 1300 mg/dl). Platelets from HC rabbits were morphologically normal by transmission electron microscopy. Survivals of 51Cr-labeled platelets from HC and non-HC rabbits were measured in HC and non-HC recipients. The results identified an intrinsic defect in the ability of HC platelets to survive in the circulation. They also confirmed previous findings of an environmental defect in HC that causes shortened platelet survival.
通过给兔子喂食添加胆固醇和花生油的饲料来诱发高胆固醇血症（HC = 高胆固醇血症或高胆固醇血症性）。在8至12周的时间间隔内，对食用高胆固醇血症饲料的动物和食用正常兔饲料的对照动物的血液进行血小板计数和体积、白细胞计数、网织红细胞计数以及血细胞比容的测定。在高胆固醇血症存在的情况下，微血小板增多症持续出现，最早在喂食饲料2周时就开始发展。微血小板增多症通常与正常血小板计数相关，但在饲料喂养后期血清胆固醇水平最高（大于1300mg/dl）时出现轻度血小板增多症。通过透射电子显微镜观察，高胆固醇血症兔子的血小板形态正常。在高胆固醇血症和非高胆固醇血症的受体中测量了来自高胆固醇血症和非高胆固醇血症兔子的51Cr标记血小板的存活情况。结果确定了高胆固醇血症血小板在循环中存活能力的内在缺陷。他们还证实了先前关于高胆固醇血症中导致血小板存活时间缩短的环境缺陷的发现。
