Cho Susie, Kim Heung-Chul, Chong Sung-Tae, Klein Terry A, Kwon Deok Ho, Lee Si Hyeock, Kim Ju Hyeon
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
Force Health Protection and Preventive Medicine, Medical Department Activity-Korea/ 65th Medical Brigade, Unit 15281, APO AP 96271-5281, USA.
Korean J Parasitol. 2020 Feb;58(1):99-102. doi: 10.3347/kjp.2020.58.1.99. Epub 2020 Feb 29.
Two-point mutations (V419L and L925I) on the voltage-sensitive sodium channel of bed bugs (Cimex lectularius) are known to confer pyrethroid resistance. To determine the status of pyrethroid resistance in bed bugs in Korea, resistance allele frequencies of bed bug strains collected from several US military installations in Korea and Mokpo, Jeollanamdo, from 2009-2019 were monitored using a quantitative sequencing. Most bed bugs were determined to have both of the point mutations except a few specimens, collected in 2009, 2012 and 2014, having only a single point mutation (L925I). No susceptible allele was observed in any of the bed bugs examined, suggesting that pyrethroid resistance in bed bug populations in Korea has reached a serious level. Large scale monitoring is required to increase our knowledge on the distribution and prevalence of pyrethroid resistance in bed bug populations in Korea. Based on present study, it is urgent to restrict the use of pyrethroids and to introduce effective alternative insecticides. A nation-wide monitoring program to determine the pyrethroid resistance level in bed bugs and to select alternative insecticides should be implemented.
已知臭虫（温带臭虫）电压敏感钠通道上的两个点突变（V419L和L925I）会导致拟除虫菊酯抗性。为了确定韩国臭虫对拟除虫菊酯的抗性状况，使用定量测序监测了2009年至2019年从韩国多个美军基地以及全罗南道木浦采集的臭虫菌株的抗性等位基因频率。除了2009年、2012年和2014年采集的少数仅具有单个点突变（L925I）的标本外，大多数臭虫被确定具有这两个点突变。在所检查的任何臭虫中均未观察到敏感等位基因，这表明韩国臭虫种群对拟除虫菊酯的抗性已达到严重程度。需要进行大规模监测，以增加我们对韩国臭虫种群中拟除虫菊酯抗性分布和流行情况的了解。基于目前的研究，迫切需要限制拟除虫菊酯的使用并引入有效的替代杀虫剂。应实施一项全国性监测计划，以确定臭虫对拟除虫菊酯的抗性水平并选择替代杀虫剂。
