Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Department of Plant Life and Environmental Science, Hankyong National University, Anseong 17580, Republic of Korea.
Food Chem. 2020 Jul 15;318:126449. doi: 10.1016/j.foodchem.2020.126449. Epub 2020 Feb 21.
Mulberry and chokeberry are rich sources of anthocyanins. In this study, the effect of the anthocyanin composition on the anthocyanin profile changes during in vitro digestion (mimicking the physiological conditions) was investigated by UHPLC-(ESI)-qTOF and UHPLC-(ESI)-QqQ. The antioxidant activity before and after in vitro digestion was elucidated. Cyanidin-3-O-glucoside and cyanidin-3-O-galactoside were dominant in mulberry and chokeberry, respectively. Moreover, the loss of cyanidin-3-O-galactoside in the chokeberry extract after digestion was greater than that of cyanidin-3-O-glucoside in the mulberry extract. After digestion, phenolic acids including protocatechuic acid and various cyanidin conjugates were newly formed because of decomposition and changes in the cyanidin-glycosides. The phenolic acid and cyanidin conjugate levels varied depending on the cyanidin glycoside sources in the colonic fraction. Finally, antioxidant activity before and after digestion was higher in the chokeberry extract than in the mulberry extract. Moreover, this activity continuously decreased until intestinal digestion but increased in the colonic fraction.
桑椹和黑果腺肋花楸富含花青素。在这项研究中，通过 UHPLC-(ESI)-qTOF 和 UHPLC-(ESI)-QqQ 研究了花青素组成对体外消化（模拟生理条件）过程中花青素图谱变化的影响。阐明了体外消化前后的抗氧化活性。桑椹和黑果腺肋花楸中的主要花青素分别为矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-半乳糖苷。此外，消化后黑果腺肋花楸提取物中矢车菊素-3-O-半乳糖苷的损失大于桑椹提取物中矢车菊素-3-O-葡萄糖苷的损失。消化后，由于原儿茶酸和各种矢车菊苷配体的分解和变化，新形成了酚酸，包括原儿茶酸和各种矢车菊苷配体。在结肠部分，由于来源于不同的矢车菊苷，酚酸和矢车菊苷配体的水平也有所不同。最后，消化前后黑果腺肋花楸提取物的抗氧化活性均高于桑椹提取物。此外，这种活性在肠道消化过程中持续下降，但在结肠部分增加。
