Jakobek Lidija, Strelec Ivica, Matić Petra
Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 18, 31000 Osijek, Croatia.
Molecules. 2025 Aug 12;30(16):3356. doi: 10.3390/molecules30163356.
Bioactive phenolic compounds released in the digestive tract have the potential to mitigate various diseases. However, they can be affected by dietary fibers. Our aim was to study the influence of β-glucan (dietary fiber) on the antiradical activity of phenolic compounds from chokeberry and its inhibition of α-amylase and α-glucosidase after digestion. These beneficial activities, helpful in many health issues connected to the digestive tract, depend on the constituents of food, such as dietary fibers, that surround these compounds and are not completely elucidated. Simulated digestion of chokeberry with or without the presence of β-glucan was conducted in vitro. The released phenolic compounds (RP-HPLC method), the antiradical activity (DPPH method), and the inhibition of α-amylase and α-glucosidase were determined after digestion. Chokeberry after gastric and intestinal digestion showed antiradical activity, and after intestinal digestion, it inhibited α-amylase and α-glucosidase. B-glucan decreased the amount of total phenolic compounds released (1800 to 1761 mg kg fw) and bioaccessibility (60 to 59%) in the stomach ( < 0.05) and small intestine (1738 to 1637 mg kg fw, 58 to 55%) ( < 0.05), decreased the antiradical activity, and weakened the enzyme inhibition. Principal component analysis clustered the released phenolic compounds and beneficial effects according to digestion with or without added β-glucan, confirming the influence of β-glucan on beneficial effects. Chokeberry polyphenols kept their beneficial effects in the stomach and small intestine in the presence of dietary fiber, which allows us to suggest that they show bioactivities even in the presence of other food constituents.
在消化道中释放的生物活性酚类化合物有减轻多种疾病的潜力。然而，它们会受到膳食纤维的影响。我们的目的是研究β-葡聚糖（膳食纤维）对消化后黑接骨木酚类化合物的抗自由基活性及其对α-淀粉酶和α-葡萄糖苷酶的抑制作用的影响。这些对许多与消化道相关的健康问题有益的活性取决于食物成分，如围绕这些化合物的膳食纤维，但其作用尚未完全阐明。在体外对有无β-葡聚糖存在的情况下黑接骨木进行模拟消化。消化后测定释放的酚类化合物（RP-HPLC法）、抗自由基活性（DPPH法）以及对α-淀粉酶和α-葡萄糖苷酶的抑制作用。黑接骨木经胃和肠道消化后显示出抗自由基活性，且在肠道消化后，它能抑制α-淀粉酶和α-葡萄糖苷酶。β-葡聚糖降低了胃中（<0.05）总酚类化合物的释放量（从1800降至1761 mg kg fw）和生物可及性（从60%降至59%）以及小肠中（从1738降至1637 mg kg fw，从58%降至55%）（<0.05），降低了抗自由基活性，并削弱了酶抑制作用。主成分分析根据有无添加β-葡聚糖的消化情况对释放的酚类化合物和有益作用进行聚类，证实了β-葡聚糖对有益作用的影响。在膳食纤维存在的情况下，黑接骨木多酚在胃和小肠中仍保持其有益作用，这使我们认为它们即使在存在其他食物成分的情况下也能表现出生物活性。
