School of Light Industry and Engineering, Qilu University of Technology, Shandong, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; School of Mechanical, Materials, and Mechatronics Engineering, University of Wollongong, NSW, Australia.
School of Light Industry and Engineering, Qilu University of Technology, Shandong, China.
Waste Manag. 2020 Mar 15;105:531-539. doi: 10.1016/j.wasman.2020.02.043. Epub 2020 Mar 5.
Preparation of biochar from industrial solid wastes is receiving increasing attention in recent years. In this paper, alkaline protease, neutral protease and collagenolytic protease are used in preliminary steps of leather production, which are expected to replace the traditional chemical agents while preserving quality of the finished leather. The protease application has remarkable positive influence towards characteristics of biochar prepared by collagen-containing solid wastes produced in preliminary steps. The enzymatic action time should be more than 3 h for complete permeation and catalysis, and the diameters of treated collagen fibers were in the range of 10 to 20 nm. The micro-cracks occurring on collagen fibers would have an obviously impact on the formation of biochar. The application of proteases reduce the pollution of traditional production through replacing traditional polluted chemicals, and the characteristics of biochars are obviously improved with good surface area and high carbon content approximately 70%. Its surface area can reach 967 m/g. These biochars contain oxygen-containing functional groups, and the oxygen content of biochars are all over 20%. The enzyme application in leather production are effective to the properties of biochars prepared by collagen-containing solid wastes. This research can serve as a basis for the preparation of biochar derived from of natural bio-wastes thereby promoting the development of biomaterials.
近年来，利用工业固体废物制备生物炭受到了越来越多的关注。在本文中，我们在皮革生产的初步步骤中使用碱性蛋白酶、中性蛋白酶和胶原酶，以期在保持皮革成品质量的同时替代传统的化学试剂。蛋白酶的应用对由胶原废物制备的生物炭的特性具有显著的积极影响。酶解作用时间应超过 3 小时，以实现完全渗透和催化，处理过的胶原纤维的直径在 10 到 20nm 之间。胶原纤维上出现的微裂纹会对生物炭的形成产生明显的影响。通过替代传统的污染化学品，蛋白酶的应用减少了传统生产的污染，并且生物炭的特性得到了明显改善，具有良好的表面积和高碳含量（约 70%）。其表面积可达 967m/g。这些生物炭含有含氧官能团，生物炭的氧含量均超过 20%。酶在皮革生产中的应用对含胶原的固体废物制备的生物炭的性能有积极影响。本研究可为天然生物废料衍生的生物炭的制备提供基础，从而促进生物材料的发展。
