Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People's Republic of China.
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 5;233:118208. doi: 10.1016/j.saa.2020.118208. Epub 2020 Feb 29.
Using Hg-promoted deprotection reaction, we have developed a new fluorescent turn-on sensor 2TS based on bithiophene fluorophore for Hg detection. The sensing mechanism of 2TS towards Hg was strongly proved by H NMR, FTIR, HRMS, UV-vis and fluorescence spectra. Remarkly, 2TS towards Hg in 100% aqueous solution shows high sensitivity with a low detection limit of 19 nM, superior selectivity and ultra-rapid response of 20 s during a wide sensing pH range from 4 to 10. Taking advantage of the excellent properties, the low-cost sensor 2TS-based filter paper/TLC test strips were fabricated for visual, immediate and quantitative detection of Hg in water, proving its applicability towards sensitive in-situ and on-site detection. Meanwhile, 2TS showed high analytical performance for Hg detection in water, seafood as well as human urine samples. Moreover, thanks to the good water solubility, negligible cytotoxicity, good biocompatibility and cell-membrane permeability, 2TS was further applied to effectively image Hg in live cells. Furthermore, the developed sensor 2TS acted as good fluorescent display material for Hg with obvious color change.
通过汞促进的脱保护反应，我们开发了一种基于联噻吩荧光团的新型荧光开启型传感器 2TS，用于 Hg 检测。2TS 对 Hg 的传感机制通过 1H NMR、FTIR、高分辨率质谱、紫外-可见和荧光光谱得到了有力证明。值得注意的是，2TS 在 100%水溶液中对 Hg 表现出高灵敏度，检测限低至 19 nM，选择性好，响应时间超快，在 pH 值为 4 至 10 的宽传感范围内仅需 20 秒。利用其优异的性能，我们制备了基于低成本传感器 2TS 的滤纸/TLC 测试条，用于水、海产品和人尿样中 Hg 的可视化、即时和定量检测，证明了其在敏感的原位和现场检测中的适用性。同时，2TS 在水样、海鲜和人尿样中对 Hg 检测表现出较高的分析性能。此外，由于良好的水溶性、可忽略的细胞毒性、良好的生物相容性和细胞膜通透性，2TS 进一步被应用于有效地在活细胞中成像 Hg。此外，所开发的传感器 2TS 作为 Hg 的良好荧光显示材料，具有明显的颜色变化。
