Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Methods Mol Biol. 2020;2121:23-36. doi: 10.1007/978-1-0716-0338-3_3.
Innate lymphoid cells (ILCs) are present in most tissues within the body but have been most extensively studied within mucosal barriers such as the lung and intestine. Isolation of immune cells from such tissues requires enzymatic digestion, and the number and composition of the cells released are dependent upon robust protocols tailored to the tissue of study. Here, detailed methodologies to isolate ILCs from various barrier sites and their draining lymph nodes (LNs) are described. Flow cytometry staining and gating strategies for identification and quantification of ILCs are then provided. Combined, these provide an efficient means to study ILCs within the small intestine lamina propria, lung, ear skin, and LNs.
先天淋巴细胞（ILCs）存在于体内大多数组织中，但在肺和肠道等黏膜屏障中得到了最广泛的研究。从这些组织中分离免疫细胞需要酶消化，并且释放的细胞数量和组成取决于针对研究组织的强大方案。在这里，描述了从各种屏障部位及其引流淋巴结（LN）分离 ILC 的详细方法。然后提供了用于鉴定和定量 ILC 的流式细胞术染色和门控策略。结合这些方法，提供了一种在小肠固有层、肺、耳皮肤和 LNs 中研究 ILC 的有效方法。
