1 Institute of Biomedical and Environmental Health Research, School of Science and Sport, University of the West of Scotland, Paisley, UK.
2 Institute of Infection, Immunity, and Inflammation and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
J Dent Res. 2018 Nov;97(12):1399-1405. doi: 10.1177/0022034518782141. Epub 2018 Jun 21.
Innate lymphoid cells (ILCs) are a population of lymphocytes that act as the first line of immunologic defense at mucosal surfaces. The ILC family in the skin, lungs, and gastrointestinal tissues has been investigated, and there are reports of individual subsets of ILCs in the oral tissues. We sought to investigate the whole ILC population (group 1, 2, and 3 subsets) in the murine gingivae and the lymph nodes draining the oral cavity. We show that ILCs made up a greater proportion of the whole CD45+ lymphocyte population in the murine gingivae (0.356% ± 0.039%) as compared with the proportion of ILCs in the draining lymph nodes (0.158% ± 0.005%). Cytokine profiling of the ILC populations demonstrated different proportions of ILC subsets in the murine gingivae versus the regional lymph nodes. The majority of ILCs in the draining lymph nodes expressed IL-5, whereas there were equal proportions of IFN-γ- and IL-5 expressing ILCs in the oral mucosa. The percentage of IL-17+ ILCs was comparable between the murine gingivae and the oral draining lymph nodes. These data suggest an enrichment of ILCs in the murine gingivae, and these ILCs reflect a cytokine profile discrepant to that of the local draining lymph nodes. These studies indicate diversity and enrichment of ILCs at the oral mucosal surface. The function of ILCs in the oral cavity remains to be determined; here, we provide a premise of ILC populations that merits future consideration in investigations of mouse models and human tissues.
固有淋巴细胞 (ILC) 是一类淋巴细胞，作为黏膜表面免疫防御的第一道防线发挥作用。皮肤、肺和胃肠道组织中的 ILC 家族已经得到研究，并且在口腔组织中也有 ILC 亚群的报道。我们试图研究鼠牙龈和口腔引流淋巴结中的整个 ILC 群体（第 1、2 和 3 亚群）。我们表明，与引流淋巴结中的 ILC 比例（0.158% ± 0.005%）相比，ILC 在鼠牙龈中的整个 CD45+淋巴细胞群体中占更大比例（0.356% ± 0.039%）。对 ILC 群体的细胞因子分析表明，在鼠牙龈和区域淋巴结中，ILC 亚群的比例不同。引流淋巴结中的大多数 ILC 表达 IL-5，而口腔黏膜中表达 IFN-γ 和 IL-5 的 ILC 比例相等。在鼠牙龈和口腔引流淋巴结中，IL-17+ILC 的百分比相当。这些数据表明，鼠牙龈中 ILC 的富集，并且这些 ILC 反映出与局部引流淋巴结不同的细胞因子谱。这些研究表明口腔黏膜表面 ILC 的多样性和富集。ILC 在口腔中的功能仍有待确定；在这里，我们提供了 ILC 群体的前提，值得在小鼠模型和人类组织的研究中进一步考虑。
