Tait Wojno Elia D, Beamer Celine A
Baker Institute for Animal Health and Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA.
Department of Biomedical and Pharmaceutical Sciences, Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, MT, USA.
Methods Mol Biol. 2018;1803:353-370. doi: 10.1007/978-1-4939-8549-4_21.
Innate lymphoid cells (ILCs) comprise a family of innate immune cells that orchestrate mucosal immune responses: initiating, sustaining, and even curbing immune responses. ILCs are relatively rare (≤1% of lymphocytes in mucosal tissues), lack classical cell-surface markers, and can be divided into three subsets (type 1-3 ILCs) based on differences in cytokine production, phenotype, and developmental pathway. Because ILCs can only be identified by combinations of cell-surface markers and cytokine production, multicolor flow cytometry is the most reliable method to purify, characterize, and assess the functionality of ILCs. Here, we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILCs from the lung.
固有淋巴细胞（ILCs）是一类固有免疫细胞家族，它们协调黏膜免疫反应：启动、维持甚至抑制免疫反应。ILCs相对稀少（在黏膜组织中占淋巴细胞的比例≤1%），缺乏经典的细胞表面标志物，并且根据细胞因子产生、表型和发育途径的差异可分为三个亚群（1-3型ILCs）。由于ILCs只能通过细胞表面标志物和细胞因子产生的组合来鉴定，多色流式细胞术是纯化、表征和评估ILCs功能的最可靠方法。在这里，我们描述了从小鼠肺中制备细胞、进行流式细胞术分析以及纯化ILCs的方法。
