Peter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, King's College London, London, UK.
Department of Inflammation Biology, School of Immunology & Microbial Sciences, King's College London, London, UK.
Methods Mol Biol. 2020;2121:199-211. doi: 10.1007/978-1-0716-0338-3_17.
Innate lymphoid cells (ILCs) are emerging as important effectors of innate immunity and play a critical role in maintaining intestinal immune homeostasis. They are tissue-residing immune cells that can be subdivided based on master transcription factor and cytokine expression, bearing striking resemblance to their CD4 T helper (Th) cell counterparts. ILCs are increasingly recognized as potential mediators of inflammatory bowel disease (IBD) providing a need to explore their functional and phenotypic differences in health vs. disease. In this chapter we outline protocols for the characterization of human ILCs and intracellular cytokine expression using flow cytometry. We include protocols for isolating human peripheral blood and colonic lamina propria mononuclear cells essential for evaluating human IBD specimens.
固有淋巴细胞 (ILC) 作为先天免疫的重要效应细胞而出现，在维持肠道免疫稳态方面发挥着关键作用。它们是组织驻留的免疫细胞，可以根据主转录因子和细胞因子表达进行细分，与它们的 CD4 T 辅助 (Th) 细胞对应物具有惊人的相似性。ILC 被越来越多地认为是炎症性肠病 (IBD) 的潜在介质，这就需要探索它们在健康和疾病状态下的功能和表型差异。在本章中，我们概述了使用流式细胞术对人 ILC 进行特征描述和细胞内细胞因子表达的方案。我们包括了分离人外周血和结肠固有层单核细胞的方案，这对于评估人类 IBD 标本是必不可少的。
