Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital & Postgraduate Medical School, Beijing, China.
Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, China.
J Clin Lab Anal. 2020 Jul;34(7):e23287. doi: 10.1002/jcla.23287. Epub 2020 Mar 9.
In this study, a new immunoassay for the simultaneous determination of pepsinogen I (PGI) and pepsinogen II (PGII) in serum based on element labeling strategy coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection was proposed.
The sandwich-type immunoassay was used to simultaneously detect PGI and PGII in serum. PGI and PGII were captured by anti-PGI and anti-PGII antibody immobilized on the magnetic beads and then banded with Eu labeled anti-PGI detection antibody and Sm labeled anti-PGII detection antibody, followed by ICP-MS detection.
The linear correlation coefficient (R ) of PGI and PGII standard curves was .9938 and .9911, with the dynamic range of 0-200 ng/mL and 0-60 ng/mL, respectively. The limit of detection for PGI and PGII was 1.8 ng/mL and 0.3 ng/mL, respectively. The average recovery was 101.41% ± 6.74% for PGI and 101.47% ± 4.20% for PGII. Good correlations were obtained between the proposed method and CLIA (r = .9588 for PGI, r = .9853 for PGII).
We established a mass spectrometry-based immunoassay for the simultaneous detection of PGI and PGII in a single analysis. The element tagged immunoassay coupled with ICP-MS detection has high sensitivity, accuracy, and specificity in clinical serum sample analysis.
本研究提出了一种基于元素标记策略结合电感耦合等离子体质谱（ICP-MS）检测的新的血清胃蛋白酶原 I（PGI）和胃蛋白酶原 II（PGII）同时测定的免疫分析方法。
采用夹心型免疫分析法检测血清中的 PGI 和 PGII。PGI 和 PGII 被固定在磁珠上的抗 PGI 和抗 PGII 抗体捕获，然后与 Eu 标记的抗 PGI 检测抗体和 Sm 标记的抗 PGII 检测抗体结合，最后进行 ICP-MS 检测。
PGI 和 PGII 标准曲线的线性相关系数（R）分别为 0.9938 和 0.9911，动态范围分别为 0-200ng/mL 和 0-60ng/mL。PGI 和 PGII 的检测限分别为 1.8ng/mL 和 0.3ng/mL。PGI 的平均回收率为 101.41%±6.74%，PGII 的平均回收率为 101.47%±4.20%。本方法与 CLIA 之间具有良好的相关性（PGI 为 r=0.9588，PGII 为 r=0.9853）。
我们建立了一种基于质谱的同时检测单个分析中 PGI 和 PGII 的免疫分析方法。元素标记免疫分析结合 ICP-MS 检测在临床血清样本分析中具有高灵敏度、准确性和特异性。
