Han Dingding, He Zeng, Zhong Rui, Zhang Xuejun, Wang Hong
Institute of Blood Transfusion, Peking Union Medical Collage, Chinese Academy of Medical Science, Chengdu 610052, Sichuan, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Feb 25;36(2):332-340. doi: 10.13345/j.cjb.190190.
In order to further improve the hemostatic performance of wound dressings, human platelet-rich plasma (PRP) containing various growth factors was introduced into chitosan solution. The silk fibroin solutions with different volume ratios (1:1, 1:3, 3:1 and 1:0) were added to improve the porosity and hemostasis of materials. The hPRP-chitosan/silk wound dressings with different ratios was prepared by freeze-drying and pure chitosan dressing was considered as the control group to study the effects of PRP and silk fibroin in different proportions on the hemostasis properties and the growth factors burst release. The hemostasis of chitosan dressing was improved by introducing PRP, but the porous structure and water absorption were not significantly improved. If silk fibroin solution was added in the ratio of 1:1, the more uniform porous structure and better hemostatic performance could be obtained. The porosity and water absorption could reach 86.83%±3.84% and 1 474%±114% respectively. In addition, the dressings with ratio of 1:1 had positive effects on reducing the burst release of growth factors on initial stage. Therefore, PRP-chitosan/silk fibroin composite dressing can become a kind of wound dressing that can achieve rapid hemostasis and promote wound healing.
为了进一步提高伤口敷料的止血性能，将含有多种生长因子的人富血小板血浆（PRP）引入壳聚糖溶液中。添加不同体积比（1:1、1:3、3:1和1:0）的丝素蛋白溶液以改善材料的孔隙率和止血性能。通过冷冻干燥制备不同比例的hPRP-壳聚糖/丝素蛋白伤口敷料，并将纯壳聚糖敷料作为对照组，研究不同比例的PRP和丝素蛋白对止血性能和生长因子爆发释放的影响。引入PRP可改善壳聚糖敷料的止血性能，但多孔结构和吸水性没有明显改善。若以1:1的比例添加丝素蛋白溶液，则可获得更均匀的多孔结构和更好的止血性能。孔隙率和吸水率分别可达86.83%±3.84%和1474%±114%。此外，1:1比例的敷料对减少生长因子在初始阶段的爆发释放有积极作用。因此，PRP-壳聚糖/丝素蛋白复合敷料可成为一种能实现快速止血并促进伤口愈合的伤口敷料。
