Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand.
Int J Pharm. 2012 Oct 15;436(1-2):141-53. doi: 10.1016/j.ijpharm.2012.06.046. Epub 2012 Jul 4.
In this study, the novel silk fibroin-based bi-layered wound dressing was developed. Wax-coated silk fibroin woven fabric was introduced as a non-adhesive layer while the sponge made of sericin and glutaraldehyde-crosslinked silk fibroin/gelatin was fabricated as a bioactive layer. Wax-coated silk fibroin fabrics showed improved mechanical properties compared with the non-coated fabrics, but less adhesive than the commercial wound dressing mesh. This confirmed by results of peel test on both the partial- and full-thickness wounds. The sericin-silk fibroin/gelatin spongy bioactive layers showed homogeneous porous structure and controllable biodegradation depending on the degree of crosslinking. The bi-layered wound dressings supported the attachment and proliferation of L929 mouse fibroblasts, particularly for the silk fibroin/gelatin ratio of 20/80 and 0.02% GA crosslinked. Furthermore, we proved that the bi-layered wound dressings promoted wound healing in full-thickness wounds, comparing with the clinically used wound dressing. The wounds treated with the bi-layered wound dressings showed the greater extent of wound size reduction, epithelialization, and collagen formation. The superior properties of the silk fibroin-based bi-layered wound dressings compared with those of the clinically used wound dressings were less adhesive and had improved biological functions to promote cell activities and wound healing. This novel bi-layered wound dressing should be a good candidate for the healing of full-thickness wounds.
在这项研究中，开发了一种新型丝素蛋白双层创面敷料。将涂蜡丝素纤维织物作为非粘性层，而由丝胶和戊二醛交联丝素/明胶制成的海绵则作为生物活性层。涂蜡丝素纤维织物的机械性能优于非涂层织物，但比商业创面敷贴网的粘性小。这通过对部分和全层创面的剥离试验结果得到证实。丝胶-丝素/明胶海绵状生物活性层具有均匀的多孔结构和可控制的生物降解性，取决于交联程度。双层创面敷料支持 L929 小鼠成纤维细胞的附着和增殖，特别是对于丝素/明胶比为 20/80 和 0.02%GA 交联的情况。此外，我们证明双层创面敷料促进了全层创面的愈合，与临床使用的创面敷料相比。用双层创面敷料处理的创面显示出更大程度的创面缩小、上皮化和胶原形成。与临床使用的创面敷料相比，丝素蛋白双层创面敷料具有更好的性能，即粘性较小，并且具有改善的生物学功能，可促进细胞活性和创面愈合。这种新型双层创面敷料应该是全层创面愈合的良好候选材料。
