Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Mashhad Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Mashhad, Iran.
J Microencapsul. 2020 Jun;37(4):305-313. doi: 10.1080/02652048.2020.1740804. Epub 2020 Mar 20.
The study was carried out to synthesise and characterise the chitosan-encapsulated genistein (CHI-En/Gen) and determine its anti-cancer and anti-angiogenic properties. The cytotoxic and anti-angiogenic activity of CHI-En/Gen was performed using MTT and chorioallantoic membrane assay. The molecular action was determined using flow cytometry and gene expression. The synthesised CHI-En/Gen was in submicron size, spherical in shape and with entrapment efficiency and loading efficiency of 76.8% (w/w) and 32.6% (w/w), respectively. The CHI-En/Gen notably inhibited the growth and proliferation of human colorectal cancer cells (HT-29) while did not affect the viability of human dermal fibroblast as normal cell. The flow cytometry and the caspase-3 gene expression analyses revealed the apoptotic cells death in the HT-29 cells. Moreover, the encapsulated genistein showed anti-angiogenic activity. The CHI-En/Gen appeared as a promising carrier for the colon delivery of genistein to be used in complementary health approaches for the cancer prevention.
本研究旨在合成并表征壳聚糖包裹染料木黄酮（CHI-En/Gen），并确定其抗癌和抗血管生成特性。采用 MTT 和鸡胚尿囊膜试验测定 CHI-En/Gen 的细胞毒性和抗血管生成活性。采用流式细胞术和基因表达测定法确定分子作用。合成的 CHI-En/Gen 呈亚微米级大小，呈球形，包封效率和载药量分别为 76.8%（w/w）和 32.6%（w/w）。CHI-En/Gen 显著抑制人结直肠癌细胞（HT-29）的生长和增殖，而对正常细胞人真皮成纤维细胞的活力没有影响。流式细胞术和 caspase-3 基因表达分析显示 HT-29 细胞发生凋亡性细胞死亡。此外，包裹的染料木黄酮表现出抗血管生成活性。CHI-En/Gen 似乎是一种很有前途的载体，可将染料木黄酮递送至结肠，用于癌症预防的补充健康方法。
