Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Endocr Metab Immune Disord Drug Targets. 2020;20(7):1110-1116. doi: 10.2174/1871530320666200309142541.
Cytokines as important mediators have a critical role in appropriate immune responses, the irregular production of which can lead to Multiple Sclerosis (MS). Proinflammatory cytokine interleukin-1 (IL-1) triggers inflammatory responses. Function and production of the cytokine are influenced by IL-1 coding gene polymorphism and those antagonists gene polymorphism.
The aim of the present study was to evaluate the possible correlation between MS and IL-1 related alleles in Azeri population of Iran.
Variable number tandem repeats (VNTR) genotypes of 150 MS patients and 220 healthy non-relative controls were determined.
In the healthy controls, genotype TT at IL-1A (-889) location was significantly higher than the MS patients (p=0.0001). However, a significant difference was not found between the two groups in genotypic/allelic frequency at IL- 1B+ 3953 location. Evaluation of the IL-1RA gene revealed that genotype 1/2, and genotype 1/3 were significantly higher in the healthy controls and MS patients, respectively. Our findings indicated that the consumption of fast-food in MS patients was significantly higher than controls (p= <0.05). Also, a considerable number of MS patients had inappropriate dieting behaviors such as not eating breakfast (p= 0.0001), and irregular eating habits (p= 0.0001).
Polymorphisms of the IL-1B genes and common alleles of IL-1RA were not considered as risk factors for MS disease. However, genotype TT at IL-1A (-889) location and the rare allele of IL-1RA3 can be a potential risk factor for the disease. Furthermore, inappropriate dieting behaviors and consumption of fast-food can increase the risk of MS.
细胞因子作为重要的介质，在适当的免疫反应中起着关键作用，其异常产生可导致多发性硬化症（MS）。促炎细胞因子白细胞介素-1（IL-1）引发炎症反应。细胞因子的功能和产生受 IL-1 编码基因多态性和那些拮抗剂基因多态性的影响。
本研究旨在评估伊朗阿塞拜疆人群中 MS 与 IL-1 相关等位基因之间的可能相关性。
对 150 例 MS 患者和 220 名非亲缘健康对照的可变数串联重复（VNTR）基因型进行了测定。
在健康对照组中，IL-1A（-889）位置的 TT 基因型明显高于 MS 患者（p=0.0001）。然而，两组在 IL-1B+3953 位置的基因型/等位基因频率之间没有发现显著差异。对 IL-1RA 基因的评估表明，健康对照组和 MS 患者分别以基因型 1/2 和基因型 1/3 为主。我们的研究结果表明，MS 患者食用快餐的比例明显高于对照组（p<0.05）。此外，相当一部分 MS 患者存在不规律的饮食习惯，如不吃早餐（p=0.0001）和不规律进食（p=0.0001）。
IL-1B 基因的多态性和常见的 IL-1RA 等位基因不能被视为 MS 疾病的危险因素。然而，IL-1A（-889）位置的 TT 基因型和 IL-1RA3 的稀有等位基因可能是该疾病的潜在危险因素。此外，不规律的饮食行为和快餐的摄入会增加 MS 的风险。
