Song Ji-Hyeon, Kim Kui-Jin, Chei Sungwoo, Seo Young-Jin, Lee Kippeum, Lee Boo-Yong
Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Republic of Korea.
J Ginseng Res. 2020 Mar;44(2):267-273. doi: 10.1016/j.jgr.2018.12.005. Epub 2018 Dec 16.
Continuous exposure to high temperatures can lead to heat stress. This stress response alters the expression of multiple genes and can contribute to the onset of various diseases. In particular, heat stress induces oxidative stress by increasing the production of reactive oxygen species. The liver is an essential organ that plays a variety of roles, such as detoxification and protein synthesis. Therefore, it is important to protect the liver from oxidative stress caused by heat stress. Korean ginseng has a variety of beneficial biological properties, and our previous studies showed that it provides an effective defense against heat stress.
We investigated the ability of Korean Red Ginseng and Korean black ginseng extracts (JP5 and BG1) to protect against heat stress using a rat model. We then confirmed the active ingredients and mechanism of action using a cell-based model.
Heat stress significantly increased gene and protein expression of oxidative stress-related factors such as catalase and SOD2, but treatment with JP5 (Korean Red Ginseng extract) and BG1 (Korean black ginseng extract) abolished this response in both liver tissue and HepG2 cells. In addition, JP5 and BG1 inhibited the expression of inflammatory proteins such as p-NF-κB and tumor necrosis factor alpha-α. In particular, JP5 and BG1 decreased the expression of components of the NLRP3 inflammasome, a key inflammatory signaling factor. Thus, JP5 and BG1 inhibited both oxidative stress and inflammation.
JP5 and BG1 protect against oxidative stress and inflammation induced by heat stress and help maintain liver function by preventing liver damage.
持续暴露于高温会导致热应激。这种应激反应会改变多个基因的表达，并可能促成各种疾病的发生。特别是，热应激通过增加活性氧的产生来诱导氧化应激。肝脏是一个重要器官，发挥着多种作用，如解毒和蛋白质合成。因此，保护肝脏免受热应激引起的氧化应激至关重要。韩国人参具有多种有益的生物学特性，我们之前的研究表明它能有效抵御热应激。
我们使用大鼠模型研究了韩国红参和韩国黑参提取物（JP5和BG1）抵御热应激的能力。然后，我们使用细胞模型确认了活性成分和作用机制。
热应激显著增加了过氧化氢酶和超氧化物歧化酶2等氧化应激相关因子的基因和蛋白质表达，但用JP5（韩国红参提取物）和BG1（韩国黑参提取物）处理可消除肝脏组织和HepG2细胞中的这种反应。此外，JP5和BG1抑制了诸如磷酸化核因子κB和肿瘤坏死因子α等炎症蛋白的表达。特别是，JP5和BG1降低了关键炎症信号因子NLRP3炎性小体成分的表达。因此，JP5和BG1抑制了氧化应激和炎症。
JP5和BG1可抵御热应激诱导的氧化应激和炎症，并通过防止肝损伤来帮助维持肝功能。
