Kim Kui-Jin, Yoon Kye-Yoon, Hong Hee-Do, Lee Boo-Yong
Department of Food Science and Biotechnology, College of Life Science, CHA University, Kyonggi 463-400, Korea.
Korea Food Research Institute, Kyonggi, Seongnam 463-746, Korea.
Molecules. 2015 Nov 10;20(11):20240-53. doi: 10.3390/molecules201119692.
Global temperature change causes heat stress related disorders in humans. A constituent of red ginseng has been known the beneficial effect on the resistance to many diseases. However, the mechanism of red ginseng (RG) against heat stress still remains unclear. To determine the effect of RG on heat stress, we examined the effect of the RG on the gene expression profiles in rats subjected to environmental heat stress. We evaluated the transcripts associated with hepatic lipid accumulation and oxidative stress in rats subjected to heat stress. We also analyzed the reactive oxygen species (ROS) contents. Our results suggested RG inhibited heat stress mediated altering mRNA expressions include HSPA1, DEAF1, HMGCR, and FMO1. We also determined RG attenuated fat accumulation in the liver by altering C/EBPβ expression. RG promoted to repress the heat stress mediated hepatic cell death by inhibiting of Bcl-2 expression in rats subjected to heat stress. Moreover, RG administered group during heat stress dramatically decreased the malondialdehyde (MDA) contents and ROS associated genes compared with the control group. Thus, we suggest that RG might influence inhibitory effect on environmental heat stress induced abnormal conditions in humans.
全球气温变化会导致人类出现与热应激相关的疾病。红参的一种成分已知对多种疾病的抵抗力具有有益作用。然而，红参（RG）对抗热应激的机制仍不清楚。为了确定RG对热应激的影响，我们研究了RG对遭受环境热应激的大鼠基因表达谱的影响。我们评估了热应激大鼠中与肝脏脂质积累和氧化应激相关的转录本。我们还分析了活性氧（ROS）含量。我们的结果表明，RG抑制热应激介导的mRNA表达改变，包括HSPA1、DEAF1、HMGCR和FMO1。我们还确定RG通过改变C/EBPβ表达减轻肝脏脂肪积累。在遭受热应激的大鼠中，RG通过抑制Bcl-2表达促进抑制热应激介导的肝细胞死亡。此外，与对照组相比，热应激期间给予RG的组显著降低了丙二醛（MDA）含量和与ROS相关的基因。因此，我们认为RG可能对环境热应激诱导的人类异常状况具有抑制作用。
