Raksha L, Shantala G B, Gangashettappa Nagaraju, Ambica R, Sinha Deepa
Department of Microbiology, Bangalore Medical College and Research Institute, Bangalore, Karnataka, India.
Department of Ophthalmology, Bangalore Medical College and Research Institute, Bangalore, Karnataka, India.
Iran J Microbiol. 2019 Oct;11(5):349-356.
Contact lenses (CLs) are increasingly being used for cosmetic or therapeutic purposes. Lack of compliance and poor hygiene towards lens care is strongly associated with microbial contamination and has been proved to result in eye infections. The present study was done to compare the microbial flora between symptomatic and asymptomatic contact lens users. The study also attempts to analyze the contact lens hygiene practices of CL users.
Six samples each were collected from both the eyes, CLs and lens cases of 40 CL users (n=240) divided into two groups based on symptoms present asasymptomatic CL users and symptomatic CL users. Organisms were identified using standard microbiological techniques.
The proportion GNB obtained in symptomatic CL users was significantly higher when compared to asymptomatic CL users (p-value= <0.003). In 56.2% eyes, the microbial flora of conjunctiva was similar to either the contact lens isolate/storage case. Enterococcal microbial keratitis was seen in one case.
There was significant microbial contamination present in CL users despite compliance to contact lens hygiene practices. There were a significant number of bacteria (p-value <0.001) present which were resistant to ampicillin, amoxicillin-clavulanate, and cefotaxime in both the groups.
隐形眼镜（CL）越来越多地用于美容或治疗目的。对镜片护理缺乏依从性和卫生习惯差与微生物污染密切相关，并且已被证明会导致眼部感染。本研究旨在比较有症状和无症状隐形眼镜使用者之间的微生物菌群。该研究还试图分析隐形眼镜使用者的镜片卫生习惯。
从40名隐形眼镜使用者（n = 240）的双眼、隐形眼镜和镜盒中各采集6份样本，根据是否有症状分为无症状隐形眼镜使用者和有症状隐形眼镜使用者两组。使用标准微生物技术鉴定微生物。
与无症状隐形眼镜使用者相比，有症状隐形眼镜使用者中革兰氏阴性菌（GNB）的比例显著更高（p值 = <0.003）。在56.2%的眼睛中，结膜的微生物菌群与隐形眼镜分离株/储存盒中的相似。有1例出现肠球菌性微生物性角膜炎。
尽管遵守隐形眼镜卫生习惯，但隐形眼镜使用者中仍存在显著的微生物污染。两组中均有大量细菌（p值 <0.001）对氨苄西林、阿莫西林 - 克拉维酸和头孢噻肟耐药。
