从阿尔伯兹省和德黑兰省的生牛奶和人体血液中分离出的非白色念珠菌属的抗真菌药敏性。
Antifungal susceptibility of non-albicans spp. isolated from raw milk and human blood in Alborz and Tehran provinces.
作者信息
Namvar Zahra, Sepahy Abbas Akhavan, Tabatabaei Robab Rafiei, Rezaie Sassan
机构信息
Depatment of Microbiology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Depatment of Medical Mycology and Parasitology, Division of Molecular Biology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Microbiol. 2019 Dec;11(6):520-526.
BACKGROUND AND OBJECTIVES
Recent reports indicate high prevalence of fungal infections due to non-albicans spp. which are present in various environments such as raw milk. The quality of milk for fungal normal flora was investigated in this study.
MATERIALS AND METHODS
A total of 262 milk samples were collected directly from milk collection tanks indesignated dairy farms and cultured in SDA media. By further analysis of grown yeasts, 69 non-albicans strains were identified. Antifungal susceptibility of the isolated species, were evaluated against amphotericin B, itraconazole, fluconazole and flucytosine. Fifty two non-albicans clinical samples isolated from human blood have been evaluated along.
RESULTS
Antifungal susceptibility evaluation in non-albicans strains isolated from milk revealed and to be 100% sensitive to flucytosine and fluconazole. showed 94% and 80% sensitivity to flucytosine and fluconazole respectively. indicated 72.72% sensitivity to fluconazole.
CONCLUSION
Evaluation of non-albicans species in raw milk and antifungal susceptibility patterns of these isolates-compare with non-albicansisolates from human blood, may help physicians to choose an appropriate medication for diseases needing long-term treatment, especially for diseases caused by local strains.
背景与目的
近期报告显示，非白色念珠菌属在生牛奶等多种环境中存在，导致真菌感染的患病率较高。本研究对牛奶中真菌正常菌群的质量进行了调查。
材料与方法
直接从指定奶牛场的牛奶收集罐中采集了262份牛奶样本，并在SDA培养基中培养。通过对生长的酵母进行进一步分析，鉴定出69株非白色念珠菌菌株。评估了分离出的菌株对两性霉素B、伊曲康唑、氟康唑和氟胞嘧啶的抗真菌药敏性。同时对从人体血液中分离出的52份非白色念珠菌临床样本进行了评估。
结果
对从牛奶中分离出的非白色念珠菌菌株进行的抗真菌药敏性评估显示，热带念珠菌和近平滑念珠菌对氟胞嘧啶和氟康唑的敏感性为100%。光滑念珠菌对氟胞嘧啶和氟康唑的敏感性分别为94%和80%。季也蒙念珠菌对氟康唑的敏感性为72.72%。
结论
对生牛奶中非白色念珠菌属的评估以及这些分离株的抗真菌药敏模式——与从人体血液中分离出的非白色念珠菌进行比较，可能有助于医生为需要长期治疗的疾病，尤其是由本地菌株引起的疾病选择合适的药物。
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