[考纳斯医科大学医院酵母菌对抗真菌剂的敏感性]

[Susceptibility of yeasts to antifungal agents in Kaunas University of Medicine Hospital].

作者信息

Skrodeniene Erika, Dambrauskiene Asta, Vitkauskiene Astra

机构信息

Department of Microbiology, Kaunas University of Medicine, Kaunas, Lithuania.

出版信息

Medicina (Kaunas). 2006;42(4):294-9.

PMID:16687901

Abstract

UNLABELLED

The aim of this study was to determine the species of yeast and their susceptibility to antifungal agents isolated from clinical specimens of patients treated in Kaunas University of Medicine Hospital.

MATERIAL AND METHODS

A total of 142 yeasts isolated from various clinical specimens of patients hospitalized in Kaunas University of Medicine Hospital were included in this study. All yeasts were cultivated on Sabouraud dextrose agar and identified using either CHROM agar or API 20C AUX system. The minimum inhibitory concentrations of fluconazole, itraconazole, and amphotericin B were determined by the ATB FUNGUS 2 agar microdilution test.

RESULTS

In all clinical specimens except blood, Candida albicans was the most frequently isolated yeast (65.5%, p<0.05). In 50% of cases, Candida parapsilosis was isolated from the blood. In vitro 15.1% of Candida albicans strains and 33.3% of Candida krusei strains were resistant to fluconazole. Twelve percent of yeast strains showed resistance to fluconazole. Nearly one-fourth of Candida albicans strains (24.7%) and 23.2% of all isolated yeast strains showed resistance to itraconazole. Almost all of fluconazole-resistant (93.3%) and 12.6% of fluconazole-susceptible yeast were found to be resistant to itraconazole (p<0.001). All of the fluconazole-resistant and 13.2% of fluconazole-susceptible strains were found to be resistant to itraconazole (p<0.001). All isolated yeast strains were susceptible to amphotericin B. Candida albicans strains were significantly frequently resistant to fluconazole than non-albicans Candida species (15.1% and 4.1%, respectively, p<0.05). Resistance of Candida albicans and non-albicans Candida species to itraconazole was the same (24.7% and 20.4%, respectively).

CONCLUSIONS

Candida albicans is the most common yeast isolated in Kaunas University of Medicine Hospital. There was determined that yeasts resistant to fluconazole were commonly resistant to itraconazole too. All isolated yeast strains were susceptible to amphotericin B.

摘要

未标记

本研究的目的是确定从考纳斯医科大学医院接受治疗的患者临床标本中分离出的酵母种类及其对抗真菌剂的敏感性。

材料与方法

本研究纳入了从考纳斯医科大学医院住院患者的各种临床标本中分离出的142株酵母。所有酵母均在沙氏葡萄糖琼脂上培养，并使用CHROM琼脂或API 20C AUX系统进行鉴定。通过ATB FUNGUS 2琼脂微量稀释试验测定氟康唑、伊曲康唑和两性霉素B的最低抑菌浓度。

结果

在除血液外的所有临床标本中，白色念珠菌是最常分离出的酵母（65.5%，p<0.05）。在50%的病例中，近平滑念珠菌从血液中分离出来。体外实验中，15.1%的白色念珠菌菌株和33.3%的克柔念珠菌菌株对氟康唑耐药。12%的酵母菌株对氟康唑耐药。近四分之一的白色念珠菌菌株（24.7%）和所有分离出的酵母菌株的23.2%对伊曲康唑耐药。几乎所有对氟康唑耐药的酵母（93.3%）和12.6%对氟康唑敏感的酵母对伊曲康唑耐药（p<0.001）。所有对氟康唑耐药的菌株和13.2%对氟康唑敏感的菌株对伊曲康唑耐药（p<0.001）。所有分离出的酵母菌株对两性霉素B敏感。白色念珠菌菌株对氟康唑的耐药率显著高于非白色念珠菌（分别为15.1%和4.1%，p<0.05）。白色念珠菌和非白色念珠菌对伊曲康唑的耐药率相同（分别为24.7%和20.4%）。