评估大豆种子提取物对寄生虫培养物和感染疟原虫小鼠的抗疟原虫及抗疟疾活性。
Evaluating Antiplasmodial and Antimalarial Activities of Soybean () Seed Extracts on Parasite Cultures and -Infected Mice.
作者信息
Nyandwaro Kevin, Oyweri Job, Kimani Francis, Mbugua Amos
机构信息
Department of Medical Laboratory Science, Jomo Kenyatta University of Agriculture and Technology Nairobi, P.O. Box 90420-80100, Nairobi, Kenya.
Kenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-0002, Nairobi, Kenya.
出版信息
J Pathog. 2020 Feb 17;2020:7605730. doi: 10.1155/2020/7605730. eCollection 2020.
BACKGROUND
parasite resistance to artemisinin-based combination therapies (ACTs) calls for development of new, affordable, safe, and effective antimalarial drugs. Studies conducted previously on soybean extracts have established that they possess antimicrobial, anti-inflammatory, anticancerous, and antioxidant properties. The activity of such extracts on parasite resistance to artemisinin-based combination therapies (ACTs) calls for development of new, affordable, safe, and effective antimalarial drugs. Studies conducted previously on soybean extracts have established that they possess antimicrobial, anti-inflammatory, anticancerous, and antioxidant properties. The activity of such extracts on.
OBJECTIVES
The aim of this study was to determine the antiplasmodial activity of soybean extracts using cultures, followed by an evaluation of safety and antimalarial activity of the extracts in ANKA strain-infected mice.
METHOD
Aqueous, methanol, and peptide extracts of soybean seeds were prepared. An evaluation of the extracts for antiplasmodial activity was carried out using two strains: D6, a chloroquine-sensitive Sierra Leone 1 strain and W2, a chloroquine-resistant Indochina 1 strain. Following the evaluation of the extracts for antiplasmodial activity was carried out using two evaluation of safety and antimalarial activity of the extracts in ANKA strain. The two extracts were tested for their therapeutic potential (curative test). The peptide extract was further assessed to determine whether it could prevent the establishment of a ANKA strain. The two extracts were tested for their therapeutic potential (curative test). The peptide extract was further assessed to determine whether it could prevent the establishment of a ANKA strain. The two extracts were tested for their therapeutic potential (curative test). The peptide extract was further assessed to determine whether it could prevent the establishment of a.
RESULTS
Peptide and methanol extracts showed good activity with IC of 19.97 ± 2.57 g/ml and 10.14 ± 9.04 g/ml and 10.14 ± 9.04 g/ml and 10.14 ± 9.04 g/ml and 10.14 ± 9.04 < 0.001) in suppression with lower doses.
CONCLUSION
The results show the presence of antimalarial properties in soybean extracts with higher curative activity when compared to the prophylactic activity. However, more research needs to be conducted on this plant to possibly establish lead compounds.
背景
疟原虫对青蒿素联合疗法（ACTs）产生耐药性，这就需要研发新的、价格可承受、安全且有效的抗疟药物。先前对大豆提取物进行的研究表明，它们具有抗菌、抗炎、抗癌和抗氧化特性。此类提取物对疟原虫对青蒿素联合疗法（ACTs）耐药性的作用需要研发新的、价格可承受、安全且有效的抗疟药物。先前对大豆提取物进行的研究表明，它们具有抗菌、抗炎、抗癌和抗氧化特性。此类提取物的活性。
目的
本研究的目的是使用培养物确定大豆提取物的抗疟原虫活性，随后评估提取物在感染ANKA株的小鼠中的安全性和抗疟活性。
方法
制备大豆种子的水提取物、甲醇提取物和肽提取物。使用两种疟原虫株对提取物的抗疟原虫活性进行评估：D6，一种对氯喹敏感的塞拉利昂1株；W2，一种对氯喹耐药的印度支那1株。在对提取物的抗疟原虫活性进行评估之后，使用两种方法评估提取物在ANKA株中的安全性和抗疟活性。对两种提取物进行治疗潜力测试（治愈性测试）。对肽提取物进一步评估，以确定它是否能预防ANKA株的建立。对两种提取物进行治疗潜力测试（治愈性测试）。对肽提取物进一步评估，以确定它是否能预防ANKA株的建立。对两种提取物进行治疗潜力测试（治愈性测试）。对肽提取物进一步评估，以确定它是否能预防。
结果
肽提取物和甲醇提取物表现出良好的活性，其半数抑制浓度（IC）分别为19.97±2.57微克/毫升和10.14±9.04微克/毫升，在较低剂量下抑制效果显著（P<0.001）。
结论
结果表明大豆提取物具有抗疟特性，与预防活性相比，其治愈活性更高。然而，需要对这种植物进行更多研究，以可能确定先导化合物。
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