Laboratório de Alimentos Funcionais, Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Biomed Res Int. 2020 Feb 19;2020:5393041. doi: 10.1155/2020/5393041. eCollection 2020.
Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 M of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate (Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, ACC, pAMPK, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of ACC (3.3-fold) and AMPK/AMPK (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of ACC (111.8-fold) and AMPK/AMPK (1.2 fold). Our results show that Resv modified PL and saturated and unsaturated FA especially in MDA-MB-231 cells, and open new perspectives to the understanding of the reported anticancer effect of Resv on these cells.
白藜芦醇（Resv）在癌症方面具有健康益处，并已被报道可调节脂质代谢的重要酶。关于其对不同乳腺癌细胞亚型中脂质组成的影响的研究很少。因此，我们研究了 Resv 处理后两种乳腺癌细胞系中磷脂（PL）、脂肪酸（FA）和脂质代谢酶的变化。MCF-7 和 MDA-MB-231 细胞分别用 80 和 200μM 的 Resv 处理 24 小时。我们通过薄层层析法用放射性标记的无机磷酸盐（Pi）分析 PL，用气相色谱-质谱法分析 FA，用 Western blot 分析脂质代谢酶（DGAT2、FAS、ACC、pAMPK 和 AMPK）。Resv 处理的 MDA-MB-231 磷脂显示磷脂酰胆碱（63%）和磷脂酰乙醇胺（35%）减少。我们观察到 Resv 处理后的 MCF-7 细胞中二十碳五烯酸（EPA）（73%）和二十二碳六烯酸（DHA）（65%）增加。有趣的是，相同的处理分别使 MDA-MB-231 细胞中的 EPA 和 DHA 增加了 50%和 90%。在 MCF-7 细胞中，Resv 增加了 ACC（3.3 倍）和 AMPK/AMPK（1.5 倍）的表达，而在 MDA-MB-231 细胞中，它抑制了 ACC（111.8 倍）和 AMPK/AMPK（1.2 倍）的表达。我们的结果表明，Resv 修饰了 PL 和饱和及不饱和 FA，尤其是在 MDA-MB-231 细胞中，并为理解 Resv 对这些细胞报道的抗癌作用提供了新的视角。
