Department of Pathology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Department of Pathology, The Third Affiliated Hospital of Bengbu Medical College, Suzhou 234000, China.
Biomed Res Int. 2020 Feb 13;2020:9791208. doi: 10.1155/2020/9791208. eCollection 2020.
NUT midline carcinoma (NMC) is a rare, poorly differentiated carcinoma defined by the presence of NUT gene rearrangement. In order to better understand the diagnostic and clinicopathologic features of this disease as they pertain to clinical practice, we have herein compiled findings pertaining to 5 cases of NMC at our institution.
Clinicopathological findings from 5 NMC cases were retrospectively reviewed, with histologic findings being reassessed and summarized accordingly. Tumor samples in the present study had been stained for markers including NUT, P63, P40, TTF-1, keratin, CK7, Syn, CD56, CgA, CD34, CD117, EGFR, and Ki-67. All cases were subjected to both fluorescence in situ hybridization (FISH) and followed up.
Of these 5 NMC cases, 2 were males and 3 were females, with ages ranging from 26 to 69 years. A total of 2 cases localized to the lung, 1 to the larynx, 1 to the maxillary gingiva, and 1 to the orbital cavity. Upon microscopic assessment, these tumors appeared as clusters of small rounded cells with interstitial neutrophil infiltration. Squamous epithelial differentiation varied between samples. NUT staining revealed strong diffuse nuclear staining in tumor cells, and FISH confirmed the presence of NUT gene translocation in these samples.
NMC is a form of highly invasive cancer that can manifest in a number of tissues including the gingiva. NMC tumors have a fairly well-defined pathological morphology, and both immunohistochemistry and FISH are valuable for NMC diagnosis.
神经母细胞瘤中脑核蛋白（NUT）中线癌（NMC）是一种罕见的、低分化癌，其特征是存在 NUT 基因重排。为了更好地了解与临床实践相关的这种疾病的诊断和临床病理特征，我们在此汇总了我院 5 例 NMC 的研究结果。
回顾性分析 5 例 NMC 患者的临床病理资料，重新评估组织学发现并进行相应总结。本研究中的肿瘤标本均进行了 NUT、P63、P40、TTF-1、角蛋白、CK7、Syn、CD56、CgA、CD34、CD117、EGFR 和 Ki-67 等标志物的染色。所有病例均进行了荧光原位杂交（FISH）检测并进行了随访。
这 5 例 NMC 患者中，2 例为男性，3 例为女性，年龄 26-69 岁。共有 2 例位于肺部，1 例位于喉部，1 例位于上颌牙龈，1 例位于眶腔。显微镜下观察，这些肿瘤呈簇状小圆形细胞，间质有中性粒细胞浸润。鳞状上皮分化程度不一。NUT 染色显示肿瘤细胞有弥漫性强核染色，FISH 证实这些样本存在 NUT 基因易位。
NMC 是一种侵袭性很强的癌症，可发生于包括牙龈在内的多种组织中。NMC 肿瘤具有相当明确的病理形态，免疫组织化学和 FISH 对 NMC 的诊断均具有重要价值。
