Wang Lei, Wang Weiwei, Zhao Hongmei, Liu Yanshan, Liu Jie, Bai Na
Department of Prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
School of Stomatology, Qingdao University, Qingdao 266000, Shandong, China.
ACS Omega. 2020 Feb 18;5(8):3996-4003. doi: 10.1021/acsomega.9b03504. eCollection 2020 Mar 3.
Although titanium is the most commonly used dental implant material, its biological aging directly leads to a lower rate of osseointegration. The aim of this study is to treat aged titanium disc surfaces using low-temperature argon-oxygen plasma (LTAOP) to obtain a more hydrophilic surface in order to enhance biological activities of osteoblasts on dental implant materials. In this study, smooth-machined titanium (SM Ti) and sandblasted and acid-etched titanium (SLA Ti) substrates were used. Aged titanium discs (SM and SLA Ti) were activated by LTAOP and the surface properties were analyzed. Osteoblasts were then seeded onto the aged and LTAOP-treated surfaces. Cell morphology, viability, and features of osteogenesis were examined. We showed that after the LTAOP treatment, the surfaces of both SM and SLA titanium substrates become more hydrophilic with a larger active oxygen species composition, whereas no obvious morphological changes were observed. Osteoblasts were found to be attached and stretched well on the surfaces of LTAOP treatment specimens. Moreover, the proliferation and osteocalcin secretion of osteoblasts on the plasma-activated titanium samples were superior to the untreated counterparts. LTAOP activation can enhance the attachment, proliferation, and mineralization of osteoblasts on the surfaces of the aged titanium substrates. This research provides a new strategy to modify the surface of titanium dental implants for improved biological functions.
尽管钛是最常用的牙科植入材料，但其生物老化会直接导致骨结合率降低。本研究的目的是使用低温氩氧等离子体（LTAOP）处理老化的钛盘表面，以获得更具亲水性的表面，从而增强牙科植入材料上成骨细胞的生物活性。在本研究中，使用了光滑加工的钛（SM Ti）和喷砂酸蚀钛（SLA Ti）基底。对老化的钛盘（SM和SLA Ti）进行LTAOP活化并分析其表面性能。然后将成骨细胞接种到老化的和经LTAOP处理的表面上。检查细胞形态、活力和成骨特征。我们发现，经LTAOP处理后，SM和SLA钛基底的表面均变得更具亲水性，活性氧成分增加，而未观察到明显的形态变化。发现在LTAOP处理标本的表面上，成骨细胞附着并伸展良好。此外，等离子体活化钛样品上成骨细胞的增殖和骨钙素分泌优于未处理的对照样品。LTAOP活化可以增强老化钛基底表面上成骨细胞的附着、增殖和矿化。本研究为改善钛牙科植入物表面的生物学功能提供了一种新策略。
