González-Blanco Carolina, Rizo-Gorrita María, Luna-Oliva Irene, Serrera-Figallo María-Ángeles, Torres-Lagares Daniel, Gutiérrez-Pérez José-Luis
Department of Oral Surgery, College of Dentistry, Seville University, Calle de Avicena s/n 41009 Seville, Spain.
Materials (Basel). 2019 May 28;12(11):1735. doi: 10.3390/ma12111735.
(1) Background. Titanium is characterized by its biocompatibility and resistance to stress and fatigue. Treatment with argon plasma may favour growth of human osteoblasts with respect to cell adhesion and proliferation. The aim of this study was to analyse the behaviour of human osteoblasts (MG-63) on Grade IV and V titanium possessing a sand-blasted, acid-etched (SLA) surface. SLA is a widely used surface treatment to create micro- and macroretentions to enhance osteoconductive properties on the surface. (2) Methods. One group of each grade of titanium was decontaminated with argon plasma and compared. On each disc, 20 × 10 cells were cultivated for morphological analysis, study of cell viability (regarding a negative control [100% viability]) and mitochondrial energy balance. (3) Results. At 24 h titanium treated with SLA showed a higher percentage of cell viability (47.3 ± 8.1%) compared to titanium IV treated with argon plasma, which presented a percentage of 79.1 ± 1.1%. Grade V titanium treated with argon plasma presented a higher viability percentage 91.3 ± 3.0% whereas nontreated Grade V titanium presented 53.3 ± 4.0%. Cells cultivated on the surfaces with an argon-plasma treatment were enlarged in comparison to non-treated discs. The cells with smaller circularity with a greater spread and spindle shape were the ones cultivated on the Grade V titanium surface. Cells seeded on treated titanium IV and titanium V, treated or not, showed higher mitochondrial activity over nontreated titanium IV. (4) Conclusions. Cells cultivated on those Grade V titanium discs that were decontaminated with argon plasma presented higher levels of cell adhesion and proliferation, lower mitochondrial damage and a higher mean cell area compared to those not decontaminated with argon plasma.
(1) 背景。钛具有生物相容性以及抗应力和抗疲劳特性。氩等离子体处理在细胞黏附和增殖方面可能有利于人成骨细胞的生长。本研究的目的是分析人成骨细胞（MG - 63）在具有喷砂、酸蚀（SLA）表面的IV级和V级钛上的行为。SLA是一种广泛使用的表面处理方法，用于形成微观和宏观固位结构，以增强表面的骨传导性能。(2) 方法。每组各等级的钛用氩等离子体进行去污处理并进行比较。在每个圆盘上培养20×10个细胞，用于形态分析、细胞活力研究（以阴性对照[100%活力]为参照）以及线粒体能量平衡研究。(3) 结果。在24小时时，与经氩等离子体处理的IV级钛相比，经SLA处理的钛显示出更高的细胞活力百分比（47.3±8.1%），经氩等离子体处理的IV级钛的细胞活力百分比为79.1±1.1%。经氩等离子体处理的V级钛的活力百分比更高，为91.3±3.0%，而未处理的V级钛的活力百分比为53.3±4.0%。与未处理的圆盘相比，在经氩等离子体处理的表面上培养的细胞有所增大。在V级钛表面培养的细胞圆形度较小，伸展性更大且呈纺锤形。接种在经处理或未处理的IV级钛和V级钛上的细胞，与未处理的IV级钛相比，显示出更高的线粒体活性。(4) 结论。与未用氩等离子体去污处理的V级钛圆盘相比，用氩等离子体去污处理的V级钛圆盘上培养的细胞表现出更高水平的细胞黏附和增殖、更低的线粒体损伤以及更大的平均细胞面积。
