Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China.
Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
FEMS Microbiol Ecol. 2020 May 1;96(5). doi: 10.1093/femsec/fiaa039.
The recently discovered nitrite-dependent anaerobic methane oxidation (n-damo) is an important methane sink in natural ecosystems performed by NC10 phylum bacteria. However, the effect of water table (WT) gradient due to global change on n-damo bacterial communities is not well studied in peatlands. Here, we analysed the vertical distribution (0-100 cm) of n-damo bacterial communities at three sites with different WTs of the Zoige peatlands in the Qinghai-Tibetan Plateau. Using an n-damo bacterial specific 16S rRNA gene clone library, we obtained 25 operational taxonomic units (OTUs) that could be divided into Groups A, B, C, D and E (dominated by A and B). The dominant group was Group B at the high (OTU14 and OTU20) and intermediate (OTU7 and OTU8) WT sites and Group A was dominant at the low WT site (OTU6 and OTU5). Using high-throughput sequencing, we observed that n-damo bacteria mainly distributed in subsurface soils (50-60 and 20-30 cm), and their relative abundances were higher at the low WT site than at the other two sites. In addition, we found that pH and nitrate were positively correlated with Group A, while total organic carbon, total nitrogen and ammonia were positively associated with Group B. Our study provides new insights into our understanding of the response of n-damo bacteria to WT gradient in peatlands, with important implications for global change.
最近发现的亚硝酸盐依赖型厌氧甲烷氧化（n-damo）是由 NC10 门细菌在自然生态系统中进行的重要甲烷汇。然而，由于全球变化导致的水位（WT）梯度对泥炭地中 n-damo 细菌群落的影响尚未得到很好的研究。在这里，我们分析了青藏高原若尔盖泥炭地三个不同 WT 位点的 n-damo 细菌群落的垂直分布（0-100 cm）。使用 n-damo 细菌特异性 16S rRNA 基因克隆文库，我们获得了 25 个可分为 A、B、C、D 和 E 组（以 A 和 B 为主）的操作分类单元（OTU）。在高（OTU14 和 OTU20）和中（OTU7 和 OTU8）WT 位点，优势组为 B 组，而在低 WT 位点，A 组占优势（OTU6 和 OTU5）。通过高通量测序，我们观察到 n-damo 细菌主要分布在亚表层土壤（50-60 和 20-30 cm）中，且在低 WT 位点的相对丰度高于其他两个位点。此外，我们发现 pH 和硝酸盐与 A 组呈正相关，而总有机碳、总氮和氨与 B 组呈正相关。本研究为我们理解 n-damo 细菌对泥炭地 WT 梯度的响应提供了新的见解，对全球变化具有重要意义。
