Elises J S, Griffiths P D, Hocking M D, Taylor C M, White R H
Department of Nephrology, Children's Hospital, Birmingham, England.
Clin Nephrol. 1988 Oct;30(4):225-9.
The protein/creatinine ratio (Up/Uc) measured in 71 early morning urine samples (EMU) correlated closely with timed overnight urine (ONU) protein excretion rates (r = 0.96). The relationship was linear throughout the entire range of normal and abnormal protein excretion, an ONU rate of 1 mg/h/m2 body surface area being proportional to an EMU Up/Uc of 5 mg/mmol. Using the Coomassie Blue dye-binding method the upper limit of Up/Uc in 377 apparently healthy children and adolescents aged 3-19 years was shown to be 20 mg/mmol. Albumin/creatinine ratios (Ua/Uc) were also determined in the 377 healthy subjects, yielding a normal working upper limit of 3 mg/mmol. Although in normal individuals studied longitudinally the day-to-day variation of both Up/Uc and Ua/Uc was appreciable, all measurements remained within the cross-sectional normal range. While the determination of Ua/Uc has a role in the study of "microproteinuria", it is comparatively costly for routine use. The measurement of the EMU Up/Uc avoids errors and difficulties associated with timed urine collection, simplifies sample handling by the laboratory and is inexpensive. In clinical practice this is the method of choice for the quantification of proteinuria in patients with renal disease.
对71份晨尿样本（EMU）进行的蛋白/肌酐比值（Up/Uc）测定结果与定时过夜尿（ONU）蛋白排泄率密切相关（r = 0.96）。在整个正常和异常蛋白排泄范围内，两者关系呈线性，每小时每平方米体表面积1毫克的ONU排泄率与5毫克/毫摩尔的EMU Up/Uc成正比。采用考马斯亮蓝染料结合法，377名3至19岁看似健康的儿童和青少年的Up/Uc上限为20毫克/毫摩尔。还对这377名健康受试者测定了白蛋白/肌酐比值（Ua/Uc），得出正常工作上限为3毫克/毫摩尔。虽然在纵向研究的正常个体中，Up/Uc和Ua/Uc的每日变化较为明显，但所有测量值均保持在横断面正常范围内。虽然Ua/Uc的测定在“微量蛋白尿”研究中具有一定作用，但用于常规检测成本相对较高。测定EMU Up/Uc可避免与定时尿液收集相关的误差和困难，简化实验室样本处理且成本低廉。在临床实践中，这是肾病患者蛋白尿定量检测的首选方法。
