构建 eGFP 标记的塞内卡病毒 A 以促进病毒中和试验和抗病毒测定。
Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay.
机构信息
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Qingdao Research Center for Veterinary Biological Engineering and Technology, Qingdao 266109, China.
出版信息
Viruses. 2020 Mar 5;12(3):283. doi: 10.3390/v12030283.
Senecavirus A (SVA), also known as Seneca Valley virus, is an emerging virus that causes vesicular disease in pigs. This virus belongs to the genus in the family . The SVA CH-LX-01-2016 was isolated from Guangdong Province of China in 2016. In this study, a recombinant SVA CH-LX-01-2016 was constructed using reverse genetics, and proven to be able to express efficiently an enhanced green fluorescent protein (eGFP) in vitro. This eGFP-tagged recombinant SVA (rSVA-eGFP) exhibited a high capacity for viral replication. Its fluorescence-tracked characteristics greatly facilitated both virus neutralization test (VNT) and antiviral assay. The rSVA-eGFP-based VNT was used to detect eight porcine serum samples, out of which four were determined to be neutralization titer-positive. Subsequently, two antiviral drugs, ribavirin and apigenin, were assayed for evaluating both effects against the rSVA-eGFP in vitro. The result showed that only the ribavirin exhibited an anti-SVA activity.
塞尼卡病毒 A（SVA），又称塞尼卡谷病毒，是一种新兴的病毒，可引起猪的水疱病。该病毒属于 科 属。SVA CH-LX-01-2016 于 2016 年从中国广东省分离得到。本研究利用反向遗传学技术构建了重组 SVA CH-LX-01-2016，并证实其能在体外高效表达增强型绿色荧光蛋白（eGFP）。该 eGFP 标记的重组 SVA（rSVA-eGFP）具有很强的复制能力。其荧光追踪特性极大地方便了病毒中和试验（VNT）和抗病毒测定。基于 rSVA-eGFP 的 VNT 用于检测 8 份猪血清样品，其中 4 份被确定为中和滴度阳性。随后，用两种抗病毒药物利巴韦林和芹菜素对 rSVA-eGFP 进行了体外抗病毒活性测定。结果表明，只有利巴韦林对 SVA 具有抗活性。
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