Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Department of Microsystems, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Sensors (Basel). 2020 Mar 5;20(5):1429. doi: 10.3390/s20051429.
A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer-poly-(2,6-di([2,2'-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on low temperature cofired ceramics technology (LTCC). The detection procedure was based on the oxidation of the substrate, i.e., in the presence of the enzyme. An alternative enzyme-free system utilized the formation of a colorful complex between Fe ions and epinephrine molecules. With the optimized conditions, the analytical performance illustrated high sensitivity and selectivity in a broad linear range with a detection limit of 0.14-2.10 nM. Moreover, the strategy was successfully used for an EP injection test with labeled pharmacological samples.
研究了一种用于检测肾上腺素 (EP) 的新型荧光传感途径。通过将酶（漆酶、酪氨酸酶）固定在聚合物-聚（2,6-二([2,2'-联噻吩]-5-基）-4-(5-己基噻吩-2-基)吡啶）上，基于低温共烧陶瓷技术 (LTCC) 开发了基于陶瓷的微型生物传感器。检测过程基于底物的氧化，即在存在酶的情况下。另一种无酶系统利用铁离子和肾上腺素分子之间形成的彩色络合物。在优化条件下，该分析策略在较宽的线性范围内表现出高灵敏度和选择性，检测限为 0.14-2.10 nM。此外，该策略还成功用于标记药理样品的 EP 注射测试。
