Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing, 100193, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing, 100193, China.
Ecotoxicol Environ Saf. 2020 May;194:110370. doi: 10.1016/j.ecoenv.2020.110370. Epub 2020 Mar 6.
Tebuconazole, a widely used fungicide, can severely disrupt the reproductive process of various organisms. In this study, we investigated the subacute effects of tebuconazole on the earthworm to fully understand its toxic implications. Herein, untargeted metabolomics, mRNA assay and biochemical approaches were adopted to evaluate the subacute effects of Eisenia fetida earthworms, when exposed to tebuconazole at three different concentrations (0.5, 5 and 50 mg/kg) for seven days. SOD enzyme activity test displayed that tebuconazole exposure interfered with the earthworms' ROS. ANN mRNA expression was down-regulated after tebuconazole exposure. H nuclear magnetic resonance (H-NMR)-based untargeted metabolomics study showed that 5 mg/kg tebuconazole exposure interfered with earthworms' metabolism. Twelve significantly changed metabolites were identified. The pathway analyses indicate that tebuconazole can disrupt the earthworm's metabolism, particularly in the AMP pathway, which impact the reproduction. This may explain the tebuconazole's mechanism of action behind the down-regulation of the expression of ANN mRNA, which is related to the earthworm's reproductive process. We comprehensively evaluated the mRNA expression, enzyme activity, and metabolomics, and acquired sufficient information for evaluating the toxicity of tebuconazole.
戊唑醇是一种广泛使用的杀菌剂，它会严重干扰各种生物体的生殖过程。在这项研究中，我们研究了戊唑醇对蚯蚓的亚急性影响，以充分了解其毒性。在此，我们采用非靶向代谢组学、mRNA 检测和生化方法来评估暴露于 0.5、5 和 50mg/kg 戊唑醇 7 天的赤子爱胜蚓的亚急性影响。SOD 酶活性测试显示戊唑醇暴露会干扰蚯蚓的 ROS。暴露于戊唑醇后，ANN mRNA 的表达被下调。基于 H 核磁共振（H-NMR）的非靶向代谢组学研究表明，5mg/kg 戊唑醇暴露会干扰蚯蚓的新陈代谢。鉴定出 12 种显著变化的代谢物。通路分析表明，戊唑醇可以破坏蚯蚓的新陈代谢，特别是在 AMP 通路中，这会影响生殖。这可能解释了戊唑醇下调与蚯蚓生殖过程相关的 ANN mRNA 表达的作用机制。我们全面评估了 mRNA 表达、酶活性和代谢组学，并获得了评估戊唑醇毒性的充分信息。
