Xu Weitao, Feng Huaming, Zhao Weiwei, Huang Chunhua, Redshaw Carl, Tao Zhu, Xiao Xin
Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang, 550025, China.
National Research Center for Geoanalysis, China Geological Survey, Beijing, 100037, China.
Anal Chim Acta. 2020 Oct 23;1135:142-149. doi: 10.1016/j.aca.2020.09.028. Epub 2020 Sep 25.
A new fluorescent chemosensor comprised of cucurbit[8]uril (Q[8]) and acridine hydrochloride (AC) has been designed and utilized for the recognition of amino acids. The AC was encapsulated by the Q[8] cavity and formed a 1:2 host-guest inclusion complex both in solution (aqueous) and in the solid-state. Whilst free AC is known to be strongly fluorescent, this strong fluorescence was quenched in the inclusion complex Q [8]-AC. This non-fluorescent complex Q[8]-AC was capable of serving as a fluorescence "off-on" probe, and was able to recognize either L-Phe or L-Trp via the competitive interaction between L-Phe or L-Trp. Moreover, the pH responsive nature of the probe allowed for the detection of basic amino acids, namely L-Arg, L-His, or L-Lys). As a result, a fluorescence method for the detection of five amino acids using a single system has been developed.
一种由葫芦[8]脲（Q[8]）和盐酸吖啶（AC）组成的新型荧光化学传感器已被设计并用于识别氨基酸。AC被Q[8]空腔包封，在溶液（水相）和固态中均形成1:2的主客体包合物。虽然已知游离的AC具有很强的荧光，但这种强荧光在包合物Q[8]-AC中被淬灭。这种非荧光复合物Q[8]-AC能够作为荧光“关-开”探针，并能够通过L-苯丙氨酸（L-Phe）或L-色氨酸（L-Trp）之间的竞争相互作用识别L-Phe或L-Trp。此外，该探针的pH响应特性允许检测碱性氨基酸，即L-精氨酸（L-Arg）、L-组氨酸（L-His）或L-赖氨酸（L-Lys）。因此，已开发出一种使用单一系统检测五种氨基酸的荧光方法。
