Agricultural Research Council-Biotechnology Platform, Private Bag X05, Onderstepoort, 0110, South Africa.
Department of Biological Sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya.
Arch Virol. 2020 May;165(5):1231-1234. doi: 10.1007/s00705-020-04582-x. Epub 2020 Mar 9.
Severe mottling symptoms were observed on Carica papaya L. in Koyonzo, Kakamega County, Kenya. Total RNA was sequenced via an RNAtag-seq workflow. Assembled contigs indicated the presence of a divergent strain of Moroccan watermelon mosaic virus (genus Potyvirus) with a complete genome length of 9,733 nt (GenBank accession no. MN418119). Additionally, the complete genome sequence of a novel member of the viral genus Allexivirus was determined (GenBank accession no. MN418120). The genome contains six open reading frames (ORFs) that show varying degrees of sequence similarity to members of the genus Allexivirus; however, it appears to lack an ORF encoding a nucleic-acid-binding homolog. The tentative name "papaya virus A" (PaVA) has been proposed for this virus.
在肯尼亚卡卡梅加县的科永佐，观察到番木瓜严重斑驳症状。通过 RNAtag-seq 工作流程对总 RNA 进行测序。组装的连续序列表明存在一种具有完整基因组长度为 9733 个核苷酸的摩洛哥西瓜花叶病毒（属 Potyvirus）的分化株（GenBank 登录号 MN418119）。此外，还确定了病毒属 Allexivirus 的一个新成员的完整基因组序列（GenBank 登录号 MN418120）。该基因组包含六个开放阅读框（ORF），它们与 Allexivirus 属的成员表现出不同程度的序列相似性；然而，它似乎缺乏编码核酸结合同源物的 ORF。该病毒被暂命名为“番木瓜病毒 A”（PaVA）。
