从黄精中分离出的一种新型马铃薯 Y 病毒的全基因组序列。
Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianum.
机构信息
Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.
College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.
出版信息
Arch Virol. 2020 Sep;165(9):2127-2131. doi: 10.1007/s00705-020-04717-0. Epub 2020 Jul 6.
The complete genome sequence of a putative novel potyvirus, tentatively named "Polygonatum kingianum virus 1" (PKgV1), infecting Polygonatum kingianum in China was determined (GenBank accession no. MK427056). PKgV1 has a genome organization that is typical of potyviruses, with a single large open reading frame (nt 123-9236) that encodes a 3037-aa polyprotein that is predicted to be cleaved into 10 mature proteins by virus-encoded proteases. Nine cleavage sites and several conserved motifs were identified in PKgV1 by comparative sequence analysis. Pairwise comparisons revealed that the PKgV1 polyprotein shares 52.0-56.2% nucleotide and 49.2-52.8% amino acid sequence identity with members of the genus Potyvirus. Phylogenetic analysis indicated that PKgV1 clustered with members of the genus Potyvirus and that it is closely related to but distinct from lettuce mosaic virus (LMV, accession no. KJ161186). These results suggest that Polygonatum kingianum virus 1 (PKgV1) is a new member of the genus Potyvirus of the family Potyviridae.
中国发现了一种暂定名为“玉竹病毒 1 号”（PKgV1）的新型马铃薯 Y 病毒属病毒的全基因组序列（GenBank 登录号：MK427056）。PKgV1 的基因组组织具有马铃薯 Y 病毒属的典型特征，其单一的大开放阅读框（nt 123-9236）编码一个 3037 个氨基酸的多聚蛋白，该多聚蛋白预计由病毒编码的蛋白酶切割成 10 个成熟蛋白。通过序列比较分析，在 PkgV1 中鉴定了 9 个切割位点和几个保守基序。序列比对显示，PKgV1 的多聚蛋白与马铃薯 Y 病毒属的成员具有 52.0-56.2%的核苷酸和 49.2-52.8%的氨基酸序列同一性。系统进化分析表明，PKgV1 与马铃薯 Y 病毒属的成员聚类在一起，与生菜花叶病毒（LMV，登录号：KJ161186）密切相关，但又有所不同。这些结果表明，玉竹病毒 1 号（PKgV1）是马铃薯 Y 病毒属的一个新成员。
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