Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Equine Vet J. 2020 Nov;52(6):794-798. doi: 10.1111/evj.13261. Epub 2020 Apr 6.
Equid herpesvirus (EHV-1) infections in horses can lead to equine herpesvirus myeloencephalopathy (EHM), characterised by neurological clinical signs. The sporadic occurrence of the disease in horse herds suggests a host genetic component. A recent study reported an association between the occurrence of EHM and genetic markers on horse chromosome 6 (ECA6).
To investigate the association of EHM with genetic host factors, especially with reference to the association reported for ECA6.
Genome-wide association study (GWAS) was conducted based on 94 horses that had EHV-1 infections and comparing the 27 developing clinical EHM to the 67 which did not.
DNA samples were tested from 94 horses for 382,529 single nucleotide polymorphisms (SNPs) with the Affymetrix Axiom 670K SNP array to identify possible associations with EHM. The data analysis included tests for basic, additive, dominant and recessive modes of inheritance, haplotype associations and runs of homozygosity (ROH).
Results from this study did not identify significant SNPs, haplotypes or ROH associations with the development of EHM following EHV-1 infections and excluded the involvement of a recessive genetic factor in the susceptibility to develop EHM.
Sample size and complex phenotype.
The results exclude the involvement of a recessive genetic factor in the susceptibility to develop clinically apparent EHM but do not have the power to exclude the involvement of other, complex host genetic factors. Furthermore, there was no association between development of EHM and genes on equine chromosome 6, as previously reported.
马疱疹病毒（EHV-1）感染可导致马疱疹病毒性脑脊髓炎（EHM），其特征是出现神经临床症状。马群中该病的散发性发生表明存在宿主遗传因素。最近的一项研究报告称，EHM 的发生与马染色体 6（ECA6）上的遗传标记之间存在关联。
研究 EHM 与遗传宿主因素的关联，特别是与 ECA6 报告的关联。
基于 94 例发生 EHV-1 感染的马进行全基因组关联研究（GWAS），将 27 例出现临床 EHM 的马与 67 例未出现 EHM 的马进行比较。
用 Affymetrix Axiom 670K SNP 阵列对 94 匹马的 382,529 个单核苷酸多态性（SNP）进行 DNA 样本检测，以确定与 EHM 相关的可能关联。数据分析包括基本、加性、显性和隐性遗传模式、单倍型关联和纯合子连续（ROH）的检验。
本研究的结果未发现与 EHV-1 感染后发生 EHM 相关的显著 SNP、单倍型或 ROH 关联，排除了隐性遗传因素在易感性方面的作用。
样本量和复杂表型。
结果排除了隐性遗传因素在易感性方面的作用，但没有排除其他复杂宿主遗传因素的作用。此外，与先前报道的 EHM 发展与马染色体 6 上的基因之间没有关联。
