Li Bin, Fan Shengxü, Yu Fukuan, Chen Ying, Zhang Shengrui, Han Fenxia, Yan Shurong, Wang Lianzheng, Sun Junming
The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
Theor Appl Genet. 2017 Jul;130(7):1467-1479. doi: 10.1007/s00122-017-2902-8. Epub 2017 Apr 7.
We constructed a high-density linkage map comprising 3541 markers developed by specific-locus amplified fragment sequencing, and identified 26 stable QTL including nine novel loci, for fatty acid composition in soybean. Soybean oil quality and stability are mainly determined by the fatty acid composition of the seed. In the present study, we constructed a high-density genetic linkage map using 200 recombinant inbred lines derived from a cross between cultivated soybean varieties Luheidou2 and Nanhuizao, and SNP markers developed by specific-locus amplified fragment sequencing (SLAF-seq). This map comprises 3541 markers on 20 linkage groups and spans a genetic distance of 2534.42 cM, with an average distance of 0.72 cM between adjacent markers. Inclusive composite interval mapping revealed 26 stable QTL for five fatty acids, explaining 0.4-37.0% of the phenotypic variance for individual fatty acids across environments. Of these QTL, nine are novel loci (qLA1, qLNA2_1, qPA4_1, qLA4_1, qPA6_1, qSA12_1, qPA16_1, qOA18_1, and qFA19_1). These stable QTL harbor three fatty acid biosynthesis genes (GmFabG, GmACP, and GmFAD8), and 66 genes encoding lipid-related transcription factors. These stable QTL and tightly linked SNP markers can be used for marker-assisted selection in soybean breeding programs.
我们构建了一个包含3541个由特异性位点扩增片段测序开发的标记的高密度连锁图谱，并鉴定出26个稳定的数量性状位点（QTL），其中包括9个新位点，用于大豆脂肪酸组成的研究。大豆油的品质和稳定性主要由种子的脂肪酸组成决定。在本研究中，我们利用200个重组自交系构建了一个高密度遗传连锁图谱，这些重组自交系来源于栽培大豆品种鲁黑豆2号和南汇早的杂交后代，以及通过特异性位点扩增片段测序（SLAF-seq）开发的单核苷酸多态性（SNP）标记。该图谱在20个连锁群上包含3541个标记，跨度为2534.42厘摩（cM），相邻标记间的平均距离为0.72厘摩。包容性复合区间作图揭示了5种脂肪酸的26个稳定QTL，解释了不同环境下单个脂肪酸表型变异的0.4%-37.0%。在这些QTL中，有9个是新位点（qLA1、qLNA2_1、qPA4_1、qLA4_1、qPA6_1、qSA12_1、qPA16_1、qOA18_1和qFA19_1）。这些稳定的QTL包含3个脂肪酸生物合成基因（GmFabG、GmACP和GmFAD8）以及66个编码脂质相关转录因子的基因。这些稳定的QTL和紧密连锁的SNP标记可用于大豆育种计划中的标记辅助选择。
