Saed Hala A R, Ibrahim Hussam M M, El-Khodery Sabry A, Youssef Mohamed A
Department of Internal medicine, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Vet Rec Open. 2020 Jan 9;7(1):e000339. doi: 10.1136/vetreco-2019-000339. eCollection 2020.
The objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.
Blood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.
Compared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.
Results of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.
本研究的目的是评估荷斯坦奶牛围产期外周血中维生素D受体（VDR）、1α-羟化酶（1α-OHase）和活化正常T细胞表达和分泌的趋化因子（RANTES）的基因表达模式。
在预计分娩日期（EDD）前3周、分娩当天和产后3周，从16头荷斯坦奶牛采集血样，使用实时PCR评估所研究基因的表达谱，并测量葡萄糖、离子钙（Ca）、甲状旁腺激素（PTH）、无机磷（P）、钠（Na）、钾（K）、氯（Cl）和镁（Mg）水平。
与EDD前3周相比，VDR基因表达在分娩当天显著下降，然后在产后3周显著增加。1α-OHase酶的基因表达以及PTH、K、Na和Cl水平在分娩当天显著增加。Ca水平在分娩当天显著下降，然后在产后3周显著增加。P水平在分娩当天显著增加，然后在产后3周显著下降。葡萄糖水平在分娩当天和产后3周显著下降。RANTES基因表达在三个不同时间点之间无显著变化。VDR基因的表达与1α-OHase酶基因的表达以及葡萄糖、PTH、P和K的血清水平呈负相关，但与血清Ca水平呈正相关。1α-OHase酶基因的表达与PTH、P和K的血清水平呈正相关，但与血清Ca水平呈负相关。
本研究结果表明监测VDR和1α-OHase酶的基因表达作为围产期代谢变化指标的重要性，表明它们是判断奶牛在此期间健康状况的候选基因。
