Yang Tao, Xie Peng, Wu Zhenzhen, Liao Yunmao, Chen Wenchuan, Hao Zhichao, Wang Yushu, Zhu Zhimin, Teng Wei
Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Front Bioeng Biotechnol. 2020 Feb 21;8:119. doi: 10.3389/fbioe.2020.00119. eCollection 2020.
Grafting bone substitute is paramount to prevent the alveolar ridge resorption after tooth extraction and facilitate the subsequent implant treatment. An ideal bone substitute should acquire the excellent osteogenic property, more importantly, possess the suitable remodeling rate in balance with bone formation and desirable clinical manageability. However, none of bone substitute is simultaneously characterized by these features, and currently, the limited remodeling property leads to the excessive waiting time before implantation. Enlightened by woven bone, the transitional tissue that is able to induce osteogenesis during bone healing could be easily remodeled within a short period and depend on the favorable injectability of hydrogel, an injectable woven bone-like hydrogel (IWBLH) was constructed in this study to address the above problems. To mimic the component and hierarchical structure of woven bone, amorphous calcium phosphate (ACP) and mineralized collagen fibril were synthesized and compounded with alginate to form IWBLHs with various ratio. Screened by physiochemical characterization and biological assays, an optimal IWBLH was selected and further explored in rat model of tooth extraction. Compared with the most widely used bone substitute, we showed that IWBLH could be easily handled to fully fill the tooth socket, perform a comparable function to prevent the alveolar bone resorption, and completely remodeled within 4 weeks. This IWBLH stands as a promising candidate for alveolar ridge preservation (ARP) in future.
移植骨替代物对于防止拔牙后牙槽嵴吸收以及促进后续种植治疗至关重要。理想的骨替代物应具备优异的成骨性能，更重要的是，在骨形成与理想的临床可操作性之间保持合适的重塑速率。然而，目前尚无一种骨替代物同时具备这些特性，且有限的重塑性能导致植入前等待时间过长。受编织骨的启发，编织骨是一种在骨愈合过程中能够诱导成骨的过渡组织，可在短时间内轻松重塑，并且依赖于水凝胶良好的可注射性，本研究构建了一种可注射的编织骨样水凝胶（IWBLH）来解决上述问题。为模拟编织骨的成分和层次结构，合成了无定形磷酸钙（ACP）和矿化胶原纤维，并与藻酸盐复合，形成不同比例的IWBLH。通过理化表征和生物学检测进行筛选，选择了一种最佳的IWBLH，并在大鼠拔牙模型中进一步研究。与目前应用最广泛的骨替代物相比，我们发现IWBLH易于操作，能够完全填充牙槽窝，在防止牙槽骨吸收方面具有类似的功能，并且在4周内可完全重塑。这种IWBLH有望成为未来牙槽嵴保存（ARP）的候选材料。
