State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia.
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russia.
Microb Drug Resist. 2020 Aug;26(8):924-933. doi: 10.1089/mdr.2019.0289. Epub 2020 Mar 9.
The objective of this study was phenotypic and genotypic characterization of antibacterial-resistant clinical strains isolated in Moscow Transplantology Intensive Care Unit in 2017-2019. Major strains among ( = 63) isolated from 30 patients were recognized as extensive drug-resistant ( = 55) pathogens, and remaining strains were recognized as multidrug-resistant ( = 8) pathogens. The beta-lactamase genes ( = 63), ( = 61), ( = 54), ( = 52), and ( = 2), as well as class 1 integrons ( = 19) carried gene cassette arrays A4 ( = 2), A1C ( = 6), BA1 ( = 9), A15A1 ( = 3), and A12 ( = 1) were identified in the strains. All strains carried four virulence genes: G, H, , and S, but two strains had additionally gene. Six known sequence types (STs) of ST395 ( = 44), ST377 ( = 3), ST307 ( = 4), ST13 ( = 2), ST39 ( = 2), ST3346 ( = 1), and a novel sequence-type ST3551 ( = 7) were identified. Phylogenetic analysis showed that ST3551 belonged to the cluster of clonal group CG147, and the remaining six STs to the another cluster consisting of four subgroups. The emergence of genetic lines carrying epidemiologically significant beta-lactamase genes ST395, ST13, ST3346, ST3551, and ST39 was the first case of detection in Russia. The emergence of novel carbapenemase-producing genetic lines in Russia highlights the global negative tendency of multidrug-resistant pathogens spread in high-technological medical centers.
这项研究的目的是对 2017 年至 2019 年在莫斯科移植重症监护病房分离的具有抗菌耐药性的临床菌株进行表型和基因型特征分析。在从 30 名患者中分离出的 63 株主要菌株中，有 55 株被认定为广泛耐药病原体，其余 8 株被认定为多药耐药病原体。在这些菌株中，共鉴定出 63 株携带 、61 株携带 、54 株携带 、52 株携带 、2 株携带 ，以及 19 株携带类 1 整合子的基因盒阵列 A4（2 株）、A1C（6 株）、BA1（9 株）、A15A1（3 株）和 A12（1 株）。所有菌株均携带 4 种毒力基因：G、H、、和 S，但有 2 株还携带 基因。共鉴定出 6 种已知的序列型（ST），包括 ST395（44 株）、ST377（3 株）、ST307（4 株）、ST13（2 株）、ST39（2 株）、ST3346（1 株）和一个新的序列型 ST3551（7 株）。系统发育分析表明，ST3551 属于克隆群 CG147 的一个聚类，而其余 6 个 ST 则属于另一个聚类，该聚类由 4 个亚群组成。携带具有流行病学意义的β-内酰胺酶基因 ST395、ST13、ST3346、ST3551 和 ST39 的遗传谱系的出现，是俄罗斯首例检测到的情况。新型碳青霉烯酶产生遗传谱系在俄罗斯的出现，突显了高技能医疗中心中多药耐药病原体传播的全球负面趋势。
