Shapovalova Valeria V, Chulkova Polina S, Ageevets Vladimir A, Nurmukanova Varvara, Verentsova Irina V, Girina Asya A, Protasova Irina N, Bezbido Victoria S, Sergevnin Victor I, Feldblum Irina V, Kudryavtseva Larisa G, Sharafan Sergey N, Semerikov Vladislav V, Babushkina Marina L, Valiullina Inna R, Chumarev Nikita S, Isaeva Guzel S, Belyanina Natalya A, Shirokova Irina U, Mrugova Tatiana M, Belkova Elena I, Artemuk Svetlana D, Meltser Aleksandra A, Smirnova Marina V, Akkonen Tatyana N, Golovshchikova Nataliya A, Goloshchapov Oleg V, Chukhlovin Alexey B, Popenko Lubov N, Zenevich Elena Y, Vlasov Aleksandr A, Mitroshina Galina V, Bordacheva Marina S, Ageevets Irina V, Sulian Ofeliia S, Avdeeva Alisa A, Gostev Vladimir V, Tsvetkova Irina A, Yakunina Maria A, Vasileva Ekaterina U, Matsvay Alina D, Danilov Dmitry I, Savochkina Yulia A, Shipulin German A, Sidorenko Sergey V
Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia.
Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia.
Antibiotics (Basel). 2024 Dec 17;13(12):1224. doi: 10.3390/antibiotics13121224.
Carbapenem-resistant (CRE) are a global health threat due to their high morbidity and mortality rates and limited treatment options. This study examines the plasmid-mediated transmission of virulence and antibiotic resistance determinants in carbapenem-resistant () and () isolated from Russian hospitals. : We performed short- and long-read whole-genome sequencing of 53 clinical isolates (48 and 5 ) attributed to 15 genetic lineages and collected from 21 hospitals across nine Russian cities between 2016 and 2022. : The plasmid analysis identified 18 clusters that showed high concordance with replicon typing, with all clusters having a major replicon type. The majority of plasmids in the IncHI1B(pNDM-MAR)/IncFIB(pNDM-Mar)-like cluster (79.16%) carried both antibiotic resistance genes (e.g., and ) and virulence factors (VFs) such as siderophore genes. We hypothesized that hybrid plasmids could play a critical role in the dissemination of antibiotic resistance genes and VFs. Comparative analyses with global plasmid databases revealed high-risk lineages of hybrid plasmids that are predominantly spread throughout Russia at present. Our findings underscore the importance of monitoring plasmid backbones for clinical management, surveillance, and infection control activities.
耐碳青霉烯类肠杆菌科细菌（CRE）因其高发病率和死亡率以及有限的治疗选择而成为全球健康威胁。本研究调查了从俄罗斯医院分离出的耐碳青霉烯类肺炎克雷伯菌（Kp）和大肠埃希菌（Ec）中毒力和抗生素抗性决定因素的质粒介导传播情况。方法：我们对53株临床分离株（48株Kp和5株Ec）进行了短读长和长读长全基因组测序，这些分离株属于15个遗传谱系，于2016年至2022年期间从俄罗斯九个城市的21家医院收集。结果：质粒分析确定了18个与复制子分型高度一致的簇，所有簇都有一种主要的复制子类型。IncHI1B(pNDM-MAR)/IncFIB(pNDM-Mar)样簇中的大多数质粒（79.16%）同时携带抗生素抗性基因（如blaNDM和blaCTX-M）和毒力因子（VFs），如铁载体基因。我们假设杂合质粒可能在抗生素抗性基因和VFs的传播中起关键作用。与全球质粒数据库的比较分析揭示了目前主要在俄罗斯传播的杂合质粒的高风险谱系。我们的研究结果强调了监测质粒骨架对于临床管理、监测和感染控制活动的重要性。
