Department of Plant Physiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Toruń, Poland.
Int J Mol Sci. 2020 Mar 6;21(5):1815. doi: 10.3390/ijms21051815.
Precise control of generative organ development is of great importance for the productivity of crop plants, including legumes. Gibberellins (GAs) play a key role in the regulation of flowering, and fruit setting and development. The major repressors of GA signaling are DELLA proteins. In this paper, the full-length cDNA of gene in yellow lupine ( L.) was identified. Nuclear-located LlDELLA1 was clustered in a second phylogenetic group. Further analyses revealed the presence of all conserved motifs and domains required for the GA-dependent interaction with Gibberellin Insensitive Dwarf1 (GID1) receptor, and involved in the repression function of LlDELLA1. Studies on expression profiles have shown that fluctuating transcript level favors proper flower and pod development. Accumulation of mRNA slightly decreases from the flower bud stage to anther opening (dehiscence), while there is rapid increase during pollination, fertilization, as well as pod setting and early development. expression is downregulated during late pod development. The linkage of activity with cellular and tissue localization of gibberellic acid (GA) offers a broader insight into the functioning of the GA pathway, dependent on the organ and developmental stage. Our analyses provide information that may be valuable in improving the agronomic properties of yellow lupine.
精确控制生殖器官的发育对于作物的生产力非常重要，包括豆类。赤霉素（GAs）在开花、结实和发育的调节中起着关键作用。GA 信号的主要抑制剂是 DELLA 蛋白。本文鉴定了黄羽扇豆（Lupinus luteus）基因的全长 cDNA。定位于核内的 LlDELLA1 聚类在第二个系统发育群中。进一步的分析表明，它具有与赤霉素不敏感矮化蛋白 1（GID1）受体进行 GA 依赖性相互作用以及参与 LlDELLA1 抑制功能所需的所有保守基序和结构域。表达谱研究表明，转录本水平的波动有利于花和荚的正常发育。从花蕾期到花药开裂（开裂），mRNA 的积累略有减少，而在授粉、受精以及荚的形成和早期发育过程中迅速增加。在荚的后期发育过程中，表达下调。GA 活性与 gibberellic acid（GA）的细胞和组织定位的联系提供了对 GA 途径功能的更广泛了解，该途径取决于器官和发育阶段。我们的分析提供了可能有助于改善黄羽扇豆农艺性状的信息。
