Li LiJuan, Yang Peng, Shi ShuYue, Zhang ZiJing, Shi QiaoTing, Xu JiaWei, He Hua, Lei ChuZhao, Wang ErYao, Chen Hong, Huang YongZhen
Institute of Bijie Test Area, Guizhou University of Engineering Science, Bijie, Guizhou 551700, China.
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China.
Animals (Basel). 2020 Mar 6;10(3):441. doi: 10.3390/ani10030441.
Extensive research has been carried out regarding the correlation between the growth traits of livestock and genetic polymorphisms, including single nucleotide polymorphisms and copy number variations (CNV). The purpose of this study was to analyze the CNV and its genetic effects of the Opn4 gene in 284 Guizhou goats (Guizhou black goat: n = 186, Guizhou white goat: n = 98). We used qPCR to detect the CNV of the Opn4 gene in Guizhou goats, and the classification results were correlated with the corresponding individual growth traits by SPSS software. The results showed that the gene had a superior effect on growth traits with multiple copy variants in Guizhou black goats, and there was a significant correlation between copy number variation sites and body length traits. Contrary to the former conclusion, in Guizhou white goats, individuals with the Normal copy number type showed superior growth traits and copy number variant sites were significantly associated with body weight traits. Therefore, the CNV of the Opn4 gene can be used as a candidate molecular genetic marker to improve goat growth traits, speeding up the breeding process of goat elite varieties.
关于家畜生长性状与基因多态性（包括单核苷酸多态性和拷贝数变异（CNV））之间的相关性，已开展了广泛研究。本研究旨在分析284只贵州山羊（贵州黑山羊：n = 186，贵州白山羊：n = 98）中Opn4基因的CNV及其遗传效应。我们采用qPCR检测贵州山羊中Opn4基因的CNV，并通过SPSS软件将分类结果与相应个体的生长性状进行关联分析。结果表明，该基因在贵州黑山羊中对具有多拷贝变异的生长性状具有显著影响，且拷贝数变异位点与体长性状之间存在显著相关性。与前一结论相反，在贵州白山羊中，正常拷贝数类型的个体表现出更优的生长性状，且拷贝数变异位点与体重性状显著相关。因此，Opn4基因的CNV可作为改善山羊生长性状的候选分子遗传标记，加快山羊优良品种的选育进程。
