Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women's Health Dexeus, Barcelona, Spain.
Cell Biology Unit, Faculty of Biosciences, Universitat Autònoma de Barcelona, Bellatera, Spain.
Zygote. 2020 Jun;28(3):217-222. doi: 10.1017/S0967199420000027. Epub 2020 Mar 11.
The aim of this study was to provide a more comprehensive understanding of 1PN intracytoplasmic sperm injection (ICSI) zygotes. To achieve this objective, we assessed whether all 1PN-derived embryos showed a similar morphokinetic pattern, and if the morphokinetic behaviour of 1PN-derived embryos was comparable with that of 2PN-derived embryos. In total, 149 1PN ICSI zygotes (study group) and 195 2PN ICSI zygotes (control group) were included in the study. Embryo development potential was evaluated in terms of blastocyst rate. Morphokinetic parameters, including the pronucleus diameter and kinetics of in vitro development, were also analyzed. Embryos derived from 1PN ICSI zygotes showed impaired development compared with 2PN-derived embryos, with blastocyst rates of 28.9% and 67.2%, respectively. The diameter of the pronucleus of 1PN zygotes was larger than that of 2PN zygotes. When compared with 2PN-derived embryos, those derived from 1PN zygotes had a visible pronucleus for a shorter time, in addition to a longer syngamy time and slower kinetic behaviour from two to nine cells. When 1PN-derived blastocysts and 2PN-derived blastocysts were compared, the developmental kinetics were similar in both groups, except for a delayed and longer duration of the compaction phase in 1PN-derived embryos. In conclusion, monopronucleated ICSI zygotes present differences in developmental capacity and morphokinetic behaviour compared with 2PN ICSI zygotes, showing particular morphokinetic parameters related to pronucleus formation. Only the 1PN ICSI-derived embryos that reached the blastocyst stage have similar morphokinetic development to blastocysts from 2PN zygotes.
本研究旨在更全面地了解 1PN 卵胞浆内单精子注射（ICSI）胚胎。为了实现这一目标，我们评估了所有 1PN 来源的胚胎是否表现出相似的形态动力学模式，以及 1PN 来源的胚胎的形态动力学行为是否与 2PN 来源的胚胎相似。共有 149 枚 1PN ICSI 受精卵（研究组）和 195 枚 2PN ICSI 受精卵（对照组）纳入研究。胚胎发育潜能通过囊胚率评估。形态动力学参数，包括原核直径和体外发育动力学，也进行了分析。与 2PN 来源的胚胎相比，1PN ICSI 胚胎的发育能力受损，囊胚率分别为 28.9%和 67.2%。1PN 受精卵的原核直径大于 2PN 受精卵。与 2PN 来源的胚胎相比，1PN 来源的胚胎原核可见时间更短，合核时间更长，从 2 细胞到 9 细胞的动力学行为更慢。当比较 1PN 来源的囊胚和 2PN 来源的囊胚时，两组的发育动力学相似，除了 1PN 来源的胚胎致密化阶段延迟且持续时间更长。总之，与 2PN ICSI 受精卵相比，单原核 ICSI 受精卵在发育能力和形态动力学行为方面存在差异，表现出与原核形成相关的特定形态动力学参数。只有达到囊胚阶段的 1PN ICSI 衍生胚胎的形态动力学发育与 2PN 受精卵衍生的囊胚相似。
